Fermentation Of Salvianolic Acid B-esterase

In this paper,I mainly studied salvianolic acid B-esterase,which was isolated,purified,and characterized,from microorganism Absidia sp.D38s strain.In addition,the hydrolyzation of salvianolic acid B-esterase was researched..In the preparation of products,the crude salvianolic acid B,which content is 10%,was purified by SP207 macroporous resin.After that the purity of Salvianolic acid B reached to 85.8%.The enzyme cultured from Absidia sp.D38 strain was purified by Sephadex G-100 and DEAE-Cellulose DE52,The molecular weight of the purified enzyme was about 37.1 kDa based on SDS-PAGE.The optimal temperature and pH of salvianolic acid-esterase were 45 ?and 5.0,respectively;and the range of stabilization was under 55 ?,pH 3.0-6.0.The Km was 73.8 mmol/L,and the Vm was 162.6 mmol/(L h).8 g salvianolic acid B was hydrolyzed by salvinolic acid B-esrerase under the conditions of 35 ? and pH 5.0.After separation by Sepabeads SP207 macroporous resin,5.63 g danshensu monomer eluted by water was obtained and its yield was 39.5%.2.98 g hydrolysate ? eluted by ethanol was obtained and its yield was 20.91%.Their purity were 99.0%and 91.2%in the HPLC method respectively.The hydrolysate ? which was one of the hydderlysis products,was known as Przewalskinic acid A by 13C NMR.