Development Of Compound Mycorrhiza Bio-fertilizer And Study Of Its Growth-promoting Effect On Pinus Tabulaeformis

In recent years the Ministry of A griculture listed microbial fertilizers and preparations as important products of biolo gical a gricultural green plantingand biological environmental protection technology,microbial fertilizers witness a rapid development.Considering that the disadvantages caused by the long-term use of chemical fertilizers,such as reduced soil fertility,soil hardening and environmental pollution,are paid more and more attention,green environmental protection has enjoyed popular support.As a result,microbial fertilizers and or ganic fertilizers become increasingly favored in the development of a griculture and forestry.Most commercially available microbial fertilizers are mainly powders in the form of bacillus for the sake of convenient storage and transportation.To this end,this study is designed to explore the embedded immobilization by combined functional mycelia and ectotrophic mycorrhizal fungi and to investigate the promotingeffect of the embedded immobilized particles on Pinus tabulaeformis seedings.1.Anta gonistic experiments were performed on the 6 strains obtained from the laboratory screenin g.Mutual anta gonistic effect was observed between strains 243 vs.331 and strains 133 vs.3532,deter mining that the compatible strain combinations were 343,3532 and 224.2.In the experiment checkingthe basic concentration and ratio of the main embeddingmaterials,it was preli minarily deter mined that the groups of SA3%-PVA1%,SA3%-PVA2% and SA3%-PVA3% exhibited the best operativity,sphericity,mass transfer rate and mechanical stren gth compared with other groups.Their viable count was 3.5×10~8cfu/g,9.5×10~8cfu/gand 1.62×10~9cfu/grespectively.with a fungi ger mination rate of 100% for all three groups.3.After adding0.5% biochar to the optimal concentration ratio SA3%-PVA3%,the spheres turned to be more re gular with mere trailin g;compared to the conditions without biochar,the mechanical stren gth was increased by 0.02% and the embeddingrate by 3%;the time required for mass transfer rate achieving1 was 5 min,which was 2 min less than that without biochar;at different crosslinkingtimes,the viable count of combination SA3%-PVA3% without biochar remained hi gher than that of combination SA3%-PVA3%+0.5% biochar.The viable count of combination SA3%-PVA3%+0.5% biochar at 30 min and 60 min were 1.61×10~9cfu/gand 0.98×10~9cfu/grespectively,which met the national requirements.For the crosslinkingtimes at 30 min and 60 min,mycorrhizal ger mination and growth status of SA 3%-PVA 3%+0.5% biochar were the best at 60 min.Consideringthe growth status of mycorrhizal fungi,the crosslinkingtime should be 60 min.4.The salt tolerance,acid and alkali resistance and antibiotic resistance of fungi in particle inoculants were superior to those in powder and liquid inoculants.However,the particle inoculants might be disinte grated upon NaCl concentration ?3%.5.All treatments exerted obvious promotingeffect on the biological characteristics and material accumulation of Pinus tabulaeformis seedings,where Treatment Compound micellar mycorrhizal bio fertilizer + biological charcoal + sheep dung showed the best result.