Studies On The Prparative Technology And The Quality Control Methods Of The Buxin Soup Based On Anti-ischemic Drug System

The thesis is divided into five chapters:The first chapter is the literature review,the second chapter is the study on the drug distraction process of Buxin soup based on anti-ischemic drug system,the third chapter is the study on the drug preparation process of Buxin soup based on anti-ischemic drug system,the fourth chapter is the study on the quality control methods of Buxin soup based on anti-ischemic drug system,the chapter five is summarized and discussed.Chapter one Literature reviewProgress on Chinese anti-ischemic mechanism of action and fill the heart and its single herb soup herbs ginseng,Poria,Polygala,Puhuang chemical composition and pharmacological effects were reviewed,a total of 85 citations.Chapter two The study on the drug distraction process of Buxin soup based on anti-ischemic drug systemSystem based on three characteristics of natural medicines,collaboration,affinity characterization,the use of the research group PK-PD-DI method technology,discovery and determined that the anti-ischemic drugs party system consisting of:two types of phenols and terpenes,which also includes flavonoids and phenolic esters two subcategories oligosaccharides,terpenes including triterpenoid saponins.Oligosaccharide esters in which the phenolic component is the main contribution component,terpenes in triterpenoid saponins as the main contribution component.And filter out the various subclasses of index components,which are flavonoids corresponding typhaneoside,oligosaccharides corresponding esters of 3,6'-two erucic acid sucrose,corresponding triterpenoid saponins ginsenoside Rb1.Based on the extraction of active substances of Buxin soup based on anti-ischemic drug system technology research,the use of orthogonal experimental design,in order to extract the total amount of terpenes and phenols as index to extract terpene phenol extraction and total amount of indicators of extraction solvent(A),solvent-fold(B),extraction time(C),extraction times(D)four factors were investigated.Terpene extract to be analyzed as an index,in addition to the factors B,the other factors A,C,D were significantly different.To extract the amount of terpene index,the best technology for the A,BbC,Dd.In total phenol extraction as an index to analyze the factors D only significant difference,there was no significant difference in the remaining factors,therefore,based on the total amount of phenol extraction as an indicator to analyze the optimum extraction of Buxin soup is AaBbC,Dd.Considering the orthogonal experiment results and the actual production and pharmacoeconomics,determine the optimum extraction of A,BbCcDd,a ethanol extracted dtimes,each chours,b times the amount of solvent usage.Where in the phenolic preparation of phenolic component indicators have been eluted completely,and does not contain terpenes index components ginsenosides Rb1;terpene preparation containing a small amount of phenolic compounds,terpenoids index components but has been completely eluted.Chapter three The study on the preparation process of Buxin soup based on anti-ischemic drug systemEffectively complement material of Buxin soup the preparation process based on anti-ischemic drugs system were studied.In total terpenes and phenols adsorption capacity and adsorption-desorption rate of the indexes on the type of resin is filtered through the measurement and analysis of six different types of macroporous resin adsorption properties of terpenes and total phenols,come D-type large macroporous adsorption of terpenes and total phenolic resins and strong desorption capability,high levels after desorption eluate terpenes and total phenols.The final choice D macroporous resin as a resin-based anti-ischemic drugs system of Buxin soup active substance.Further to the content and terpenes and total phenol adsorption analysis was mainly on indicators D macroporous resin preparation studies were carried out to optimize the adsorption conditions,cleaning conditions,elution conditions,including the concentration of the sample solution,sample volume and the resin volume ratio is higher than the resin column diameter,adsorption velocity,maximum sample volume;washing times the amount of washing flow rate;elution solvent,elution flow rate,elution times the amount of other parameters investigated to determine the best resin purification process:fill the Buxin soup extract water dispersed into concentration N2(in crude drug content)of the sample solution by D macroporous resin adsorption,the sample volume and the resin volume ratio to T4,high resin column diameter ratio of G2,adsorption flow rate of FV1;after adsorption finished with SB2-fold resin elution volume of water cleaning,washing flow rate of SV2;washing impurity completed after elution with X5 ethanol 4 times the volume of resin,elution rate is XV2,to collect X5 ethanol eluent solvent recovery,eluting with TX3 ethanol three times the volume of the resin,the elution flow rate of XV2,collecting TX3 ethanol eluent,solvent recovery,vacuum drying,make up the Buxin soup based on effective parts and terpene phenolic drugs effective part of the system.Buxin soup effective active substances and phenols terpenes preparation materials were verified.Preparation and content of three batches of samples was measured is phenolic active substance phenolic content of 43.00%on average,terpenoids effective substance terpene content of 73.31%on average,Buxin soup active substances in the average rate of total phenols transfer value of 73.80%,total terpene average of 66.84%transfer rate,Where 3,6'-two mustard acyl transfer rate of sucrose average of 83.63%,typhaneoside transfer rate with an average of 62.37%,ginsenoside Rb1 transfer rate with an average of 58.78%,indicating that Buxin soup terpene phenolic active substances and substance reasonably effective,stable extraction enrichment processes.A comparative study of Buxin soup water extract,alcohol extract,and enrichment efficacy.After intravenous injection of rat pituitary vasopressin sublingual acute myocardial ischemia model,select the serum creatine kinase(CK)and lactate dehydrogenase(LDH)as a measure of myocardial ischemic injury biochemical indicators,through data analysis concluded that the model control rats CK,LDH was significantly higher than the control group,indicating that the sublingual intravenous pituitrin acute myocardial ischemia model was successful.Buxin soup water extract group,alcohol extract group and the preparation of rats CK,LDH were significantly lower than those in the control group(P<0.01),Buxin soup description of each extract on rat model of myocardial injury have a therapeutic effect;Buxin soup alcohol extract and enrichment rats CK,LDH were significantly lower than the water extract group(P<0.01),described alcohol extract and enrichment group efficacy was significantly higher than the water extract group;Buxin soup alcohol extract group and the preparation of rats CK,LDH showed no significant difference between the two can be drawn on the efficacy in the treatment of myocardial ischemia considerable.But the contrast Buxin soup alcohol extract group needs gavage 0.113g/only a day,while enrichment group just a day gavage 0.0244g/only,fill the heart can be seen only soup preparation group will be able to use less amount achieve optimal efficacy.Chapter four The study on the quality control methods of Buxin soup based on anti-ischemic drug systemEstablish a visible spectrophotometry Buxin soup extract based on anti-ischemic drugs system,method for the determination of phenolic active substances Buxin soup total phenols,and ferric chloride-ferricyanide color reaction conducted a methodological study.With 3,6'-two mustard acyl sucrose as reference to calculate the regression equation:y=79.049x+0.116 R 2=0.9991(n=6),indicate that 3,6 '-bis mustard acyl sucrose in the concentration range 0.00172mg/mL?0.01032 mg/mL and absorbance showed a good linear relationship.3,6'-two erucic acid sucrose average recovery was 102.74%,RSD=1.31%(n=6).Determination of three batches of samples results show that this method is accurate,reliable,good stability,can be used as an effective way to Buxin soup phenolic active substances and active substances terpenes total phenols quality control.A high performance liquid chromatography Buxin soup based on anti-ischemic drugs extract system,Buxin soup of phenolic active substances in the index soup ingredients 3,6 '-two mesons typhaneoside acyl sugar and quantitative methods of HPLC which,3,6'-two mesons acyl sugar regression equation:y=2251992x-15518 R 2=1.0000(n=6);typhaneoside regression equation:y=2007015x-6000.9 R2=0.9999(n=7).Experimental results show that 3,6'-two meson acyl sucrose 0.1236?g?0.7416?g,typhaneoside within 0.06867?g?0.48069?g mass range and peak area showed a good linear relationship.Three batches of Buxin soup extract,Buxin soup phenolic active substance 3,6'-two mesons typhaneoside acyl sucrose and determine the content,the results show that the method is accurate,simple,stable and can be used as fill the Buxin soup 3,6 ' extracts and phenolic active substances-Determination method Two meson group of sucrose and typhaneoside.Establish a visible spectrophotometry system of Buxin soup extract based anti-ischemic drugs,Buxin soup terpenes method for the determination of effective substances terpenes,and vanillin-perchloric acid color reaction method school visits.Ginsenoside Rbl as reference to calculate the regression equation y=18.125x-0.0074 R 2=0.9988(n=7),indicates ginsenosides Rbl in the concentration range 0.008mg/mL?0.056mg/mL with a good linear relationship between absorbance.Ginsenosides Rbl average recovery rate was 99.84%,RSD=1.67%(n=6).Determination of three batches of samples results show that this method is accurate,reliable,good stability,can be used as an effective way of Buxin soup of effective substances and terpene phenolic substances terpenes effective quality control.HPLC method for the determination of Buxin soup based on anti-ischemic drugs systems,Buxin soup terpenes effective substances index components Rb1 from quantitative HPLC method,which Rbl from the regression equation:y=173330x-12289 R 2=0.9918(n=6).Experimental results show that ginsenoside Rbl in 1.24?g?7.44?g mass range and peak area showed a good linear relationship.Three batches of Buxin soup extract,Buxin soup soup phenolic active substances to determine the content of ginsenosides Rbl,the results show that the method is accurate,simple,stable and can be used as fill the Buxin soup extract and terpene active substances ginsenosides Rbl the method for the determination.Chapter five Summarized and Discussed1 Established extraction process of Buxin soup active substance based on anti-ischemic drugs systems.2 Established preparation process of Buxin soup phenolic effective part and terpene effective part,and fill the Buxin soup extract,alcohol extract,enrichment compared the efficacy study found that Buxin soup rich set contains a very good efficacy.3 Established a quality control methods of Buxin soup phenolic effective part and terpene effective part based on anti-ischemic drugs systems.