The Study Of The Aminated Nanodiamond Fluorescent Tracer In Vivo

Biocompatible and biodegradable hydrogels received significant attention for biomedical applications such as control drug delivery and medical during past decades,a quantitative assess of degradation of hydrogels in vivo is of great importance especially for assessing degradation rate to design customized hydrogel controllable,which can be applied to newly generated tissues.By fluorescence-related tracking and monitoring can reach a way to quantitatively depicting the degradation profile of hydrogel in a real-time and non-invasive manner.However,the fluorescent imaging commonly used is limited,such as intrinsic photo bleaching of organic fluorophores and uncertain perturbation of degradation induced by the changes of the structure of hydrogel.To address these problems,we employed photo luminescent Nanodiamond with low photo bleaching,good biocompatibility as fluorescent indicator mixed with hydrogels for real-time and non-invasive monitoring of the degradation of injectable hydrogels in vitro/in vivo.The results were as follows:1:By mixing the tamarind gum and gallic acid,the article synthesize a kind of phosphate buffered solution gel,which has good coagulation stability.measuring the fluorescence intensity of gel in different slow-release time,when the pH=7,the fluorescence decay minimum and stability of the highest.2:We combined the aminated nanodiamond with compound gel,by measuring the fluorescence intensity of different slow release time to evaluate the degradation PBS gel marked by aminated nanodiamond.We test the cytotoxicity of aminated nanodiamond,phosphate buffered solution gel and the mixture of both to the HepG-2 cell and LO2 cell by MTT experiment.The results showed that minimum cell survival rate was 75.2%under experiment concentration which is of low toxicity for cell.By the toxicity test in mice in vivo,the LD50 of ethanol phase of aminated diamond is 1.85 g/kg.by the fluorescence density in mice to observe the degradation of aminated nanodiamond with compound gel timely.In the paper,we provide the first example of using photo luminescent Nanodiamond as fluorescent indicator for real-time and non-invasive visual degradation in vitro/in vivo.Then explore the nature of the mixture of aminated nanodmond and hydrogel.