| Yuxue bi capsule,Chinese patent medicine,with huoxue huayu,tongluo analgesic effect.Composed of frankincense,myrrh,radix clematis,safflower,salvia miltiorrhiza,radix cyathulae,ligusticum chuanxiong,angelica,tablet turmeric,rhizoma cyperi,astragalus,11 herbs,it can be used to treat bi syndrome caused by blood stasis.Because of its clinical curative effect is exact,and toxic side effect is small wait for an advantage and wide welcome.From the current clinical application,yu xue bi capsule dosage is larger,in order to improve consumer perception,reduce dosage,improve patient compliance.In this study,process optimization study will be carried out to optimize the process method to reduce the amount of ointment.This research mainly aimed at the present situation of Chinese native medicine preparation process research is importance link in addition to the process of research,the purpose is to reduce the amount of pure cream of liquids,and determination of the key indicators of drug efficacy,this study choose whole party extraction method respectively,oil and water extraction method comparing with the original method,the whole party will extract radix cyathulae,salvia miltiorrhiza and grilled astragalus extract,together with the other ingredients extract on TLC identification,determination of Dan phenolic acids content,B=sodium,transfer rate calculation,to ensure that the content conforms to the standard,eventually the whole extraction process compared to the original minus 39.19%cream.With oil and water extraction process is extracted,the entire prescription,is divided into two parts,part of medicinal materials to extract the volatile oil,including rhizoma ligustici wallichii,incense,turmeric na,decoction time for 4 hours,other herbs in water extract on TLC identification,determination of Dan phenolic acids content,B=sodium content conforms to the standard,oil and water extraction cream 25.95%reductioaAnd optimize the original method and purification,to extract the centrifugal water extract-alcohol precipitation in the process;the study of the process,resin purification process,centrifugal purification method of using small centrifuge are studied in removing impurity,centrifugal process for centrifugal liquid temperature is 80?,70? and 60? respectively,centrifugal time 15 minutes,20 minutes,30 minutes,high revolutions of 4500 r/min,3000 r/min and 1500 r/min,the orthogonal experiment,the extract of TLC identification,determination of Dan phenolic acids content,B=sodium,the computation rate and ensure that content is up to the standard.Aqueous alcohol sink in the method the liquid extract liquid temperature 60? and 40?,20? respectively,alcohol sink respectively for 12 hours,18 hours,24 hours a day,add alcohol concentration were 95%,85%,75%,and the orthogonal experiments and purification,extract of TLC identification,determination of Dan phenolic acids content,B=sodium,transfer rate calculation,to ensure that the content conforms to the standard.Resin purification method of resin using HPD500(polarity),D-101(nonpolar),HP20(medium polarity),the proportion byl:4,1:3,2-1,solvent elution with70%ethanol,80%ethanol 90%ethanol,orthogonal experiment was carried out to determine the best purification scheme,extract of TLC identification,determination of Dan phenolic acids content,B=sodium,transfer rate calculation,to ensure that the content conforms to the standard.Eventually determine oil and water extraction,the whole party extraction Dan phenolic acids content,B=sodium content conforms to the standard,and has a significant reduction in cream,will ultimately determine the two methods to enlarge pilot tests,thin layer chromatography was carried out on the finished product identification,determination of Dan phenolic acids content,B=sodium,transfer rate calculation,measured the indexes accord with a standard,and reduce paste volume stability,for the subsequent preparation process optimization and quality standards,laying a foundation for the development of new drugs. |
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