| Lactarius delicious is a genus of the genus Lactarius,also known as pine mushroom,which is a rare edible fungus resource.The color of the capillaris mushroom cap is yellow-gray,and the cap will have green scars due to injury during picking.Edible mushroom polysaccharide has strong anti-tumor activity,and edible fungus polysaccharide has higher safety of taking compared with traditional anti-tumor drugs.The main findings are as follows:(1)In this paper,L.edodes was studied,and the polysaccharides were extracted from the lentinan,and the extraction,purification,composition and in vitro antioxidant activity of L.edodes polysaccharides were systematically studied.Using the yield of polysaccharide as an index,the response surface method was used to optimize the process of hot water extraction of L.edodes polysaccharide.The second polynomial was obtained by Design-expert software.The yield of yam mushroom polysaccharide(Y)corresponds to the extraction temperature(A).The regression equation for extraction time(B)and water to material ratio(C)is:Y=-42.548+0.8432 A+4.63650 B+0.1519 C-0.22 AB-0.00025 AC+0.0035 BC-0.004265A2-0.4315 B2-0.002065 C2.The analysis results show that the order of influence of various factors on the yield of polysaccharides is: time> material-liquid ratio> temperature.The optimal extraction scheme was obtained with an extraction temperature of 89.52 °C,an extraction time of 3.23 h,and a water-to-feed ratio of 34.09 ? 1.The yield of the polysaccharide under this condition was 5.42%.In this paper,the best decolorization process conditions are decolorization temperature 70 °C,decolorization time 120 min,pH 9 and hydrogen peroxide dosage 8%.Under the optimum conditions,the decolorization rate of polysaccharide was 86.06%,and the polysaccharide retention rate was 69.33%.(2)After pulverizing the lentinus,the lentinan polysaccharide was extracted by hot water extraction,and then purified by deproteinization and DEAE-52 column to obtain purified polysaccharides SRG-1 and SRG-2(SRG-1 is white fibrous)Powder,SGR-2 was light yellow powder),Sephadex G-75 column purified SRG-1 to obtain secondary purified polysaccharide SRGS-1,and dialyzed.The average molecular weight of the purified L.edodes polysaccharide SRGS-1 was determined by gel exclusion chromatography to be 87,768 Da.It can be seen from the mid-infrared spectrum of SRGS-1 that the absorption peak at 3314 cm-1is the stretching vibration absorption peak of-OH,and the absorption peak at 2942 cm-1 and2900 cm-1 is the stretching vibration absorption peak of CH.The absorption peak at 1650cm-1 is the bending vibration absorption peak of-OH,and the absorption peak at 1369 cm-1is the deformation absorption peak of CH2.At 1065 cm-1,the angular absorption vibration absorption peak of the alcoholic hydroxyl group-OH is obtained.There is a characteristic absorption peak of ?-type glycosidic bond at 870 cm-1,indicating that the polysaccharide of Pinus yunnanensis is ?-polysaccharide.(3)Antioxidant experiments showed that SRGS-1 has the ability to scavenge hydroxyl radicals,super anion radicals and DPPH radicals.In this experiment,the total reducing power,hydroxyl radical scavenging ability,superoxide anion scavenging power and DPPH scavenging force of SRGS-1 were found to have natural antioxidant capacity,and the antioxidant capacity with the increase of concentration Enhanced.The antioxidant capacity of L.edodes polysaccharides increased with the increase of polysaccharide concentration,showing a dose-dependent manner.The polysaccharide component of Lactarius glutinosa is resistant to the effective scavenging of superoxide anion radicals and can be used to prevent damage caused by superoxide radicals under pathological conditions. |
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