Fabrication Of PGS@PLLA Core-shell Fibers For Wound Dressing And Drug Releasing

Biomimetic scaffolds made by synthetic materials are usually used to replace the natural tissues aimed at speeding up the skin regeneration.The structure of nano-/microfibers fabricated by coaxial electrospinning is similar to dermal extracellular matrix(ECM).So it is widely used to apply in the field of tissue regeneration,wound dressing,drug carrier and biocatalysis because of the character of high specific surface area,porosity and the core-shell structure of fibers.In this study,a flexible and cytocompatible poly(glycerol sebacate)@poly-L-lactic acid(PGS@PLLA)fibers with a core-shell structure was fabricated by coaxial electrospinning,where the shell PLLA was used to be a skeleton with pores on the fibrous surface.The PGS@PLLA fibers with core-shell structure not only can be a kind of wound dressing to speed up wound healing,but also can use as drugs carrier of Gatifloxacin to investigate the release efficiency.In the paper,the fibrous morphology with the pores on the surface of the prepared fibers was observed by SEM.The core-shell microstructure of PGS@PLLA fibers was confirmed by TEM and Laser Scanning Confocal Microscopy(LSCM).FTIR,DSC,TG and XRD were used to character the chemical properties of the core-shell fibers.The mechanical and degradable property were investigated by tensile machine and degradation tests,respectively.In addition,the wettability of PGS@PLLA core-shell fibers was tested by water contact angle.The cytotoxicity test and the wound model of mouse were used to demonstrate the wound healing.Gatifloxacin(Gat)was loaded into the core fiber layer to prepare the drug-loaded PGS@PLLA@Gat core-shell fibers.Finally,the drug release efficiency of the core-shell fibers was tested by the ultraviolet spectrophotometer(UV)and the drug release response of the PGS@PLLA@Gat core-shell fibers in different environments was investigated.As a result,the prepared fibers exhibited the strong ability to repair tissues of the skin wound,where the stability of cell security and proliferation,and the lower inflammatory response were all superior to those of pure PLLA scaffold.It's worth noting that the percentage of skin tissue was regenerated by 95%within 14 days,which suggests the potential application for electrospun-based synthetic fibrous scaffolds on wound healing.Moreover,the PGS@PLLA core-shell fibers showed a sustained drug release effect within 600 min and responsibility of temperature.At 37?,the drug release efficiency could reach to 90%,while the efficiency was 70%at 20?.