The Studies On Cutting Propagation And Physiological And Biochemical Of Buddleja Lindleyana

Buddleja lindleyana is an excellent ornamental plant,which belongs to Buddleja L of Angiospermae.The seed is not easy to root and germinate in nature,and can not form community aggregation.Cuttage is a common way to inherit good traits and improve reproductive efficiency.Under the background of field investigation and related research reading,Effects of different concentrations of different concentrations of hormones,age of cuttings and different cutting seasons on rooting of the rooting and the blank of physiological and biochemical studies in the cuttage propagation of the roth,Using random single factor experiment to study the cutting propagation technology and physiology and biochemistry of B.lindleyana.This study aims to provide more effective cutting propagation technology and theoretical direction of B.lindleyana and to accelerate the breeding and application of B.lindleyana.The results of the related research are as follows:1.Research on cutting propagation technique of B.lindleyana.The result is summarized as follows:(1)Rooting type is the root of cortex.(2)The species,concentration,matrix,age and cutting time of different hormones have significant influence on the rooting of cuttings.The optimal cuttage is to use the annual and half lignification branches as the cuttings,the matrix is loess:perlite(1:1)or vermiculite:perlite(1:1),the hormone is IBA200mg/L treatment of 1h,and the cuttage time is autumn.The root rate was 84%.(3)The rooting index has no significant difference between the low concentration treatment in 2h and the high concentration treatment in 2min.The promotion of rooting is not obvious.2.Research on the physiological and biochemical effects of different concentrations of exogenous hormone IBA on the cuttage rooting process of the B.lindleyana.The result is summarized as follows:(1)There are three stages in the process of the formation of adventitious root of B.lindleyana,which is the early stage of adventitious root,the initial stage of adventitious and the occurrence of adventitious root.(2)The values of soluble sugar,soluble protein,POD,SOD and MDA in the cuttings were all presented significantly different,the degree of influence on physiological index changes was ranked as IBA200mg/L>IBA500mg/L>CK.The deeper the influence,the better the rooting effect.(3)Rooting rate was positively correlated with soluble sugar content,soluble protein content,POD activity and SOD activity and negative correlated with malondialdehyde content.(4)The trend of soluble sugar and soluble protein content is almost the same,all of which are,falling rising and falling.The content was decreased during the early stage and gradually increased during the forming stage and occurrence stage and drop off after the peak.The change of the content of the two nutrients was greater than that of CK with the treatment of IBA,exogenous hormone treatment can accelerate the consumption rate of nutrients.(5)The activity change trend of POD is similar to that of SOD,both of them are ascending and descending.With the treatment of IBA200mg/L,the activity of POD was always higher than that of IBA500mg/L and CK.Exogenous IBA treatment significantly increased POD and SOD activity.(6)MDA activity rises first and then drops.The content of MDA was basically lower than that of CK with the treatment of IBA,and hormone treatment can significantly reduce MDA content.3.The result of different time to take the branch is summarized as follows:The content of nutrients and oxidase activity in August 31,September 15 and September 30 was higher than October 15,October 30 and November 14 while the MDA content is on the opposite.This may be related to the seasonal differences in physiological and biochemical indexes of the cuttings.