Study On Ex Situ Conservation And Cultivation Techniques Of Rhododendron Latoucheaeis

As a kind of alpine cuckoo,Rhododendron latoucheaeis very goodlandscaping tree speciesand has yet to be developedto study.R.latoucheae's whole plant has a high appreciation and medicinal value,its plant type is good,fast growth,resistance to pruning,strong adaptability,colorful andlong flowering period and so on,by the majority of love and talent in the pursuit.Loved by the vast number of people love flowers.The over-cutting by people and the deterioration of the environment have led to a significant reduction in the number of wild alpine cuckoo.Therefore,to strengthen the protection of antlers Rhododendron resources,to ensure its survival and reproductioneffectively,which is of great significance for the protection of biodiversity and the introduction and promotion of varieties.In order to protect it better,we need to understand the alpine cuckoo and R.latoucheae systematically.In recent years,there are many experts and scholars has made breakthroughsin the different varieties of alpine cuckoo cultivation and protection.of breakthroughs in the progress of the hope that on this basis.It is hoped thata better understanding of what method of cultivation is most effective for breeding antlers.In order to better protect this plant,we need to systematically understand the alpine azalea,and use the ex situ conservation to do better and more systematic understanding of the azalea.In recent years,there are a lot of experts and scholars in the different varieties of Alpine azalea cultivation and protection of breakthrough progress,hoping to further understand the basis of this method of cultivation,the most effective breeding of azalea.In order to preserve the resources of Rhododendron latoucheae,and to provide a feasible theoretical basis and supporting technical means for large-scale artificial cultivation for R.latoucheae,woody flower antlers R.latoucheae was selected as the paper's study object.Guided by the theories of forest ecology and artificial forest cultivation,the key techniques of ground protection transplanting,asexual propagation techniques,phenological regularity,photosynthetic characteristics,cutting propagation technology and tissue culture technologyof the wild resources of R.latoucheae were studied systematically.The main research contents are as follows:The Key Techniques of Conservation and Tansplantation ofR.latoucheae was studied.The phenology observation experiment of R.latoucheae from 50 to 300 years old after transplant 2 yearswas carried out.Photosynthetic characteristics of the 1 year,3 years,5 yearsand 9 years of R.latoucheae,3 years of Rhododendron delavayi and 3 years of Rhododendron fortunei were measured and compared.The cutting experiment was made with 25 year old R.latoucheae twig.2 years of R.latoucheae wild tree were choosed for the mother tree.A series of tissue culture experiments were carried out in 3?5 cm buds collected in 3,4 and 5 months.The main results are as follows:(1)Gs diurnal variation curve,Tr day curve and Pn curve were basically the same to show as asymmetric bimodal curve,and Ci diurnal curve was shown as a single peak curve in different transplanting ages of R.latoucheae,R.delavayi and R.fortunei after transplanting 3 yesrs.The net photosynthetic rate of R.latoucheae was sorted by transplanting for 1 year>transplanting for 9 years>transplanting for3 years.The net photosynthetic rate of different varieties of Rhododendron was:R.fortunei>R.latoucheae>R.delavayi.(2)The trend of light response curve was consistent in different transplanting ages of R.latoucheae,R.delavayi and R.fortunei after transplanting 3 yesrs.The photosynthetic capacity of R.fortunei at different transplanting ages were sorted by transplanting for 1 year>transplanting for 9 years>transplanting 3 years,different varieties of alpine rhododendron photosynthetic capacity of the order:R.fortunei>R.latouc heae>R.delavayi.(3)In the cutting propagation experiment of R.Aatoucheae,the results showed that half-lignified branches were used as cuttings got the highest survival rate with 300mg/L GGR as rooting agent,soaked in cuttings for 2h,and cut in 95%shade net.(4)Selecting the stem tip as explant got the best effect.In the process of explant pretreatment,leaveing half of the bud and washing samples with washing powder water to reducing browning rate.The optimal sterilization time for the explants was:alcohol immersion 30s + 0.1%mercury sterilization was 14min.(5)Antler startup medium and optimum hormone concentration combinationof R.latoucheaewas:WPM + 0.8mg/L TDZ + 0.6mg/LIBA + 20 g/L sucrose + 6 g/L agar.The induction rate was 74.44%after 45d,the average bud length was 0.87cm,and the increment coefficient was 1.73.(6)The optimum sucrose concentration of R.latoucheae was 30g/L,and the average bud length of 20d was 2.2cm.The optimum medium and optimum hormone concentration for adventitious bud increment were WPM+0.4 mg/L TDZ + 0.3 mg/IBA+30g/L sucrose +6g/L agar.The bud growth rate was 2.5cm after 30d,and the increment coefficient was 3.6.(7)The optimum medium and optimum hormone concentration for callus induction and differentiaiionof R.latoucheae were:WPM+0.4mg/LTDZ+0.2mg/LIBA+30g/L sucrose +6g/L agar.The differentiation rate was 13.33%after 30d,the average bud length was 0.37,and the increment coefficient was 2.33.(8)The optimum medium and the best hormone concentration combination for callus induction and differentiation of R.Aatoucheae stem section were WPM+ 0.4mg/LTDZ+0.2mg/LIBA+30g/Lsucrose +6g/Lagar.The differentiation rate was 90%after 30d,the average bud length was 0.73cm,and the increment coefficient was 5.33.(9)The rooting medium and the optimum hormone concentration combination of R.latoucheaewere 1/2MS+2.0mg/LNAA+0.4mg/LIBA+30g/L sucrose +6g/L agar.The rooting rate was 53.3%after 60d.