Study On Tissue Culture And Metabolic Regulation Of Gallic Acid In Callus Of Acer Ginnala Maxim.

In the thesis, mature seeds of Acer ginnala Maxim. were taken as culture material to establish the regeneration system in vitro through adventitious buds differentiating directly from explants and callus pathways. Then culture technique of metabolite regulation of gallic acid content in callus was studied. The results were as follows:1,The establishment of regeneration systemMature seeds sprouted on WPM medium supplement with 1.0 mg/L 6-BA. Young seedling were germinated with radicle cut as explants, the adventitious buds were obtained after 4 weeks culture following incubation on WPM medium supplement with 0.01 mg/L TDZ and 0.1 mg,/L6-BA.The frequency of adventitious buds was as high as 93.3%. The average proliferation number of buds per explant was 3.12. Using stems as explants, callus were easily induced on WPM medium supplement with 0.002-0.01 mg/L TDZ and 0.1mg/L6-BA after 3 weeks' culture. The frequency of callus induction was 98.0%.On WPM medium containing 0.01 mg/L TDZ and 0.1 mg/L 6-BA, 42% of callus differentiated and the mean number of buds per callus was about 10.The buds developed rooted on WPM medium with 0.3 mg/L IBA and formed plantlets, 89% of which survived and grew when transplanted to greenhouse.2, metabolites regulation culture of gallic acid in callusThe system for gallic acid accumulation in callus was established: The highest yield of. gallic acid was obtained when callus cultured in the NT medium and add 0.01mg/LTDZ and 0.1mg/L 6-BA with pH 5.8 and sources concentration 20 g/L, and the highest yield was 245.3/ bottle. Compared with MS, WPM, B5, N6, NT was not only to be best suitable for callus growth, but also was ideal to the gallic acid accumulation; the suitable concentration of sucrose was 20g/L, the gallic acid content was 3.34%, the yield of gallic acid was 245.3mg/ bottle; The suitable pH value of medium was 5.8. When pH 5.8, gallic acid content was 3.05% and the production was 133.8mg/bottle; and Little satisfactory result had been received on different kinds of lights (red, yellow, blue and green) and ultraviolet.