Study On The Rapid Propagation Technology Of New Raspberry Strain

Raspberry is a small fruit tree rich in various nutrients.In the production of raspberry,tillers are mainly used to propagate.This method has the disadvantages of easy mixing of varieties and easy infection of seedlings without establishing a special breeding garden.‘DNS15’,‘DNS20’,‘DNS40’,‘Polka’ are raspberry varieties introduced from Russia and Poland,especially the latter three varieties are early-ripening,large-fruit autumn-fruiting varieties.Establishing rapid propagation technology of these varieties are important for accelerating their promotion.The pollution,low multiplication coefficient and poor rooting of these cultivars in tissue culture were studied in this experiment.The conclusions were as follows:(1)Gram staining and molecular biology identification showed that the contaminated bacteria in raspberry tissue culture were Bacillus safensis,Bacillus pumilus,and Burkholderia cenocepacia.(2)Preliminary screening of bacteriostatic reagents showed that the most effective reagents and minimum effective bacteriostasis concentration for Bacillus safensis and Bacillus pumilus were: benzylpenicillin sodium for injection was 70 ten thousand units/L and ampicillin was 20 mg/L.And the better reagents and minimum effective bacteriostasis concentration for Burkholderia cenocepacia were: kanamycin was 75 mg/L,streptomycin sulfate for injection was12.5 ten thousand units/L,and zhi peiling was 2 ml/L.Carboxybenzylpenicillin,jinggangmycin and oxytetracycline were ineffective for the three bacteria.(3)The results of single factor treatment of antibiotics and zhipeiling showed that the best treatment was zhipeiling 2.5 ml/L,the longest bacteriostasis time was 15 days,followed by ampicillin 30 mg/L and streptomycin sulfate for injection 6.25 ten thousand units/L,and the longest bacteriostasis time was 7 days and 5 days,respectively.The results of two-factor treatment of antibiotics showed that the best treatment was ampicillin 30 mg/L+streptomycin sulfate for injection 6.25 ten thousand units/L.The longest bacteriostasis time was 6 days.(4)The results of disinfectant treatment showed that 0.2%Hg Cl2 disinfection for 2minutes was the best,and the bacteriostasis time was 10 days.The results of re-inoculation treatment showed that the plants after disinfection could be subcultured without contamination for 12 months at the longest.(5)The optimum multiplication basic medium of ‘DNS20’ was MS,the optimum multiplication medium was MS+0.6 mg/L 6-BA+0.2 mg/L NAA,the multiplication coefficientwas 5.2,the optimum rooting medium was 1/2 MS+0.1 mg/L IBA+0.02 mg/L NAA,and the rooting rate was 63.3%.(6)The optimum multiplication basic medium of‘DNS40’was WPM+1.6 g/L NH4NO3,the optimum multiplication medium was WPM+1.6 g/L NH4NO3+0.6 mg/L 6-BA,and the multiplication coefficient was 3.7.The optimum rooting medium was 1/2MS+0.02 mg/L NAA,and the rooting rate was 70%.(7)The optimum multiplication basic medium of ‘Polka’ was 1/2MS+1/2WPM,the optimum multiplication medium was 1/2MS+1/2WPM+0.6 mg/L 6-BA+0.2 mg/L IAA,the multiplication coefficient was 3.4.The optimum rooting medium was 1/2MS+0.02 mg/L NAA+0.30 mg/L IAA,the rooting rate was 80%.