| Dairy cattle industry is one of the three pillars industries related of the national economy and the people’s livelihood.Bovine mastitis is one of the most important diseases that will reduce the economic benefits of dairy farming.Antibiotic therapy has been known as the first choice in the treatment of bovine mastitis for a long time.However,the problems such as antibiotic residues and the emergence of drug-resistant strains are increasingly serious,which not only increases the complexity of disease prevention and treatment,but also endanger consumers’ health.Studies have found that a variety of pathogenic microbes can cause bovine mastitis.Mastitis has very high regional difference in epidemiology.Therefore,it is very important to study the incidence regularity of mastitis in a certain area for the prevention and treatment of dairy cow mastitis.Linqu County,which belongs in Weifang city of Shandong Province,is a traditional animal husbandry and dairy farming county.There are 37 thousand and 300 dairy cows in the county.The purpose of this study is to investigate the epidemiology of Dairy Cow Subclinical Mastitis in Linqu County of Shandong Province,isolate the main pathogenic bacteria,and detect the bacterial resistance,so as to provide evidence for prevention and treatment of dairy cow mastitis.First of all,according to the different feeding methods and management mode,we selected 4 herds in Linqu county which contains more than 200~1500 representative farms.Epidemiological investigation of bovine mastitis was detected by CMT method.Meanwhile,we analyzed the incidence of subclinical mastitis of dairy cows in the region.The results showed that the incidence subclinical mastitis of dairy cows in different fields is different.The incidence of pasture is lower than that of dairy farm.The dairy farm which had bed exhibited lower rate than that without bed.The incidence of subclinical mastitis was higher in summer and lower in winter.The incidence of subclinical mastitis is higher in 6-10 months.Subclinical mastitis can be occurred in all the parities and the incidence rate increased with increasing parity.Secondly,we randomly selected 30 mastitis milk samples from each dairy farm,with a total of 120 samples.We isolated and identified the main pathogenic bacteria causing the mastitis.The results showed that the major pathogenic bacteria are mainly Streptococcus,Streptococcus epidermidis,Staphylococcus aureus,Streptococcus,and Escherichia coli.Meanwhile,the main pathogens of different farms were different.In addition,the majority of cases were characterized by the mixed infection of several bacteria.These results suggest that we should take different control for measures according to main pathogens in various farms.Finally,the antimicrobial susceptibility tests were conducted in the main pathogenic bacteria,including Streptococcus agalactiae and Staphylococcus aureus,in order to analyze their antibiotic resistance and guide the medication in the process of prevention and treatment of mastitis.The results showed that Streptococcus agalactiae is sensitive to ciprofloxacin and vancomycin and resistant to ampicillin,tetracycline and erythromycin;Staphylococcus aureus is sensitive to vancomycin,doxycycline,rifampin,and resistant to ampicillin,tetracycline and penicillin.In conclusion,the epidemiological survey of bovine subclinical mastitis was conducted in Shandong County of Linqu province.We isolated the main pathogenic bacteria and detected the drug resistance of bacteria to grasp the incidence of mastitis,which can guide drug treatment.Finally,through the comprehensive analysis of the incidence of different types of dairy farms,we guide the farm to improve and control the disease incidence. |
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