| Objective:In this experiment,we investigated low-frequency electroacupuncture in blank group rats,PCOS model rats(99.99%dehydroepiandrosterone(DHEA)injection model)and high fat diet rats in acupuncture points(Guanyuan,Zhongji,Zigong,Sanyinjiao).By examining the OGTT and serum lipid metabolism indices(HLD-C,LDL-C,TG,TC)in each group of rats,we investigated whether low-frequency electroacupuncture can improve abnormal glucose and lipid metabolism in rats with PCOS and high-fat diet.Detection of liver oil red "O" staining and RT-PCR detection of liver tissue in each group(GSK3-β,INSR,GLUT1,GLUT4,PPARa,PPARy,SREBF1,SREBF2)mRNA content,to explore low-frequency electro-acupuncture Can improve the metabolic abnormalities(PCOS and obesity)model rat liver tissue sugar,lipid metabolism related factors,clarify that low-frequency electricity for PCOS patients and obese women with glucose and lipid metabolism disorders have a regulatory role and then play a therapeutic role.Methods:21-day-old clean female rats(SD)(body weight 50±5g)were selected,and then 48 female SD rats were randomly divided into 6 groups(n=8)by orientation.The blank group,blank acupuncture group,PCOS group,PCOS acupuncture group,high-fat diet group,high-fat diet acupuncture group;model:1 After randomized PCOS group,PCOS acupuncture group group began to give Rats were subcutaneously injected into the neck of the rats(DHEA(60mg/100g)was dissolved in 0.2 ml of soybean oil for injection),and the rest of the rats were injected with 0.2 ml of soybean oil for injection daily;2 the high-fat diet group after randomization,Rats in the high-fat diet acupuncture group were given a high-fat diet immediately after grouping,and rats in the remaining groups were given a basal diet.After the model was established 21 days later,the model was evaluated.The model was evaluated as follows:1 The general condition of the rats was the weight change of the rats;2 The estrus cycle of the rats in each group was observed;3 The HE staining of the ovaries of the rats.Low-frequency electro-acupuncture intervention:After evaluation of the model(21 days after modeling),low-frequency electro-acupuncture intervention was performed with continuous waves at a frequency of 2 Hz,1 time/day,5 days/week,and 5 consecutive weeks.15 minutes/time in the first week,20 minutes/time in the second and third weeks,and 25 minutes/time in the fourth and fifth weeks.The points are Guanyuan,Zhongji,Zigong,Sanyinjiao.Materials:The vaginal smears were taken 2 weeks before the end of the low-frequency electroacupuncture intervention.The estrus cycle of the rats in each group was observed.After entering the second regular estrous cycle,the eyelids were taken to the neck and sacrificed,and the rats were quickly taken out.Rat liver tissue was weighed and rapidly placed in liquid nitrogen and frozen and transferred to a-70℃ freezer for preservation.Detection index:OGTT,serum lipid metabolism related indicators,ovarian HE staining,liver tissue oil red"O" staining and RT-PCR detection of liver tissue in each group of rats(GSK-3β,INSR,GLUT1,GLUT4,PPARα,PPARγ The relative expression of SREBF1,SREBF2)mRNA.Results:1.The rats were generally in good condition during feeding,with stable vital signs,moisturized coat,good activity,and good drinking water.There was a difference in body weight between the blank acupuncture group and the blank group,PCOS group and PCOS acupuncture group,high fat diet group and high fat diet acupuncture group,indicating that acupuncture group and PCOS acupuncture were visible after acupuncture treatment.The body weights of rats in the acupuncture group and high-fat diet group were significantly lower than those in the blank acupuncture group,PCOS acupuncture group,and high-fat diet acupuncture group.Therefore,after the intervention of low-frequency electroacupuncture,there were The effect of weight loss in rats.2.PCOS rat model evaluation:In this experiment,the model group rats showed estrus cycle disorder on the estrous cycle.PCOS group compared with the blank group,PCOS acupuncture group and PCOS group were statistically significant There was a significant difference between the groups;in the HE staining of ovarian sections,the experimental results showed that the ovaries of the PCOS group showed polycystic changes.3.Low-frequency electroacupuncture studies on glucose and lipid metabolism in various rat serum:OTTG:PCOS acupuncture group compared with PCOS group at 30 min,PCOS acupuncture group compared with PCOS group at 60 min,high fat diet acupuncture group and high fat Compared with the dietary group,the PCOS acupuncture group and PCOS group had statistical significance(p<0.05).Serum lipid metabolism(HLD-C,LDL-C,TG,TC):Compared with the PCOS group,the PCOS acupuncture group in HLD-C showed a statistically significant difference between the high-fat diet acupuncture group and the high-fat diet group(p<0.05);Compared with blank group and PCOS group in TG,PCOS acupuncture group and PCOS group had statistical significance(p<0.05);TC showed blank group compared with PCOS group,PCOS acupuncture group and PCOS group There was a statistically significant(p<0.05)comparison between the blank group and the PCOS group in the LDL-C group.There was a statistically significant difference between the PCOS acupuncture group and the PCOS group(p<0.05).4.Low-frequency electroacupuncture was used to study the metabolism of glucose and lipid in the liver tissue of each group:The mRNA expression of GSK3-β,INSR,GLUT1,GLUT4,PPARa,PPARy,SREBF1,and SREBF2 in liver tissue.Compared with PCOS group,GSK3-β blank group had statistically significant difference between PCOS acupuncture group and PCOS group(p<0.05).In the INSR blank group and PCOS group,PCOS acupuncture group had higher fat than PCOS group.Dietary acupuncture group was statistically significant compared with high-fat diet group(p<0.05);GLUT1,GLUT4 blank group compared with PCOS group,PCOS acupuncture group compared with PCOS group,high-fat diet acupuncture group and high-fat Compared with the diet group,there was a statistically significant difference(p<0.05).Compared with the control group,the PCOS acupuncture group and the PCOS group had a statistically significant difference between the high-fat diet group and the high-fat diet acupuncture group.p<0.05),PCOS gamma PCOS group compared with the blank group,PCOS acupuncture group and PCOS group had statistically significant(p<0,05);SREBF1,SREBF2 PCOS group compared with the blank group,PCOS acupuncture group and PCOS In group comparisons,there was a statistically significant difference in the high-fat diet group and the high-fat diet group(p<0.05).Conclusion:1.Low-frequency electroacupuncture was used to regulate glucose and lipid metabolism in metabolic syndrome(PCOS and obesity)rats.2.Low-frequency electroacupuncture can improve the treatment of polycystic ovary syndrome by improving the abnormality of liver and glucose metabolism in rats with polycystic ovary syndrome.3.Based on the results of this study,it is presumed that low-frequency electroacupuncture may improve the reproductive function of patients with polycystic ovary syndrome by improving liver lipid metabolism related factors in patients with polycystic ovary syndrome. |
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