Effects Of Quercetin On Human Oral Keratinocytes During Gingival Wound Healing

Purpose/Aims: The width of keratinized gingiva plays an important role in esthetic and functional outcomes of dental implants.Lack of keratinized gingiva may lead to poor oral hygiene and greater soft-tissue recession.Quercetin,because of its significant antioxidant,anti-inflammatory,and antimicrobial activity,is useful for tissue regeneration.This study aimed at assessing the potential of quercetin in promoting gingival wound healing and re-epithelialization.Materials and Methods: Human oral keratinocytes(HOK)were detected in the absence or presence of test substances.The CCK-8 kit was used to assess cell viability and proliferation capacity.Re-epithelization was assessed using a keratinocyte monolayer scratch assay;Cell migration was monitored using Transwell chambers.Porphyromonas gingivalis lipopolysaccharide(Pg.LPS)was used for mimicking the inflammatory situation.m RNA expression of some inflammatory cytokines(IL-1β,TNF-α),cell adhesion molecules(Integrin-α6,Integrin-β4),and growth factors(TGFβ-1,TGFβ-3)were estimated using quantitative RT-PCR;Protein contents of TGFβ-1 and TGFβ-3 were evaluated by ELISA.Results: Multiplex analysis revealed that quercetin enhances HOK proliferation via an upregulation of adhesion molecules(Integrin-α6,Integrin-β4).Additionally,reepithelialization rate was significantly greater in the presence of quercetin than in the control(P < 0.01).Furthermore,small dose of quercetin increases both m RNA and protein levels of TGFβ-3 under basal and wound conditions without affecting TGFβ-1 production.Expression of proinflammatory cytokines were downregulated by quercetin treatment.Conclusion: Quercetin promotes re-epithelialization by α6β4 integrin activation and modulate wound healing process by balancing TGFβ mediated pathways.