| ObjectiveTo observe the situation which the healing-wound nutrition oilpromotes the big mouse's injured skin to repair, and to discuss tothe mechanism which the healing-wound nutrition oil promotes the bigmouse's injured skin to repair and reduces to form the scar thatthe big mouse's injured skin heals.Method1. The healing-wound nutrition oil preparation:①The yolk oilpreparation: The ripe egg-yolk, except the moisture content,fries are dry the oil, spare.②Fatty tissue extract preparation:The male 4 months' age healthy pigs, one cow, one sheep, cuts andtakes out the back fatty tissue, the antiseptic 0.01mol/Lphosphate buffer solution (PBS, pHT.2) carries on 10 times ofdilution, ice bath homogenate, 1000r/min centrifugation for 5minutes, absorbs the supernatant to degerm through themicroporous membrane, cry opreservation 20℃.③The sesame oilheats up the fining to filter. Sesame oil, yolk oil according to10: 1 proportion; the pig, the cow, the sheep with draw the thingaccording to 3: 3: 5 proportions join in the oil, examinationcharacter, osmotic pressure and pH value.2. Experimental animal source: The common big mouse was provided byLiaoning medical college animal experimentation center.3. The mold making: 45 big mice backs respectively create two openentire thick skins injured area, the diameter is 3cm, the depthreaches the muscular layer.4. Handling method: 45 big mice stochastically divide into threegroups, the blank group (A group): Externally applies thephysiologic saline; the VEGF externally applied group (B group):In the big mouse's injured area spreads the gelatin spongeincluding 40ng VEGF; the healing-wound nutrition oil group (Cgroup): Wipes the healing-wound nutrition oil about lmm thick, the aseptic dressing wraps up the injured area. To observe thebig mice injured area healing and forming the scar in each groupthrough the vital sign, the injured area partialobservation(including healing time, healing rate, epidermisesgrowth width of each group, the scar area of comparing after makingthe mold latter 28 days),the histology observation (includingcollagen fiber volume, capillary count, under electricity mirrorcell ultrastructure observation), the immunity tissue chemistrymethod to detect CDK4 and the RT-PCR method to detect VEGFmRNA.Results1. The ordinary circumstances 45 bigmice completely survive, eachgroup of injured areas only have the little courage anduprightness to see pout the spot, the not obvious infection sign,the injured area, the digestive tract and secrete the urethraexcreta not to see the hemorrhage.2. The healing-wound nutrition oil group to heal the time short, theepidermis grows quickly, after the scar area is smaller, surpassesother pairs group, the VEGF group, the healing-wound nutritionoil group and the blank group compares has significance thedifference, (p＜0.01), the healing-wound nutrition oil group andthe VEGF group compares has significance the difference,explained the healing-wound nutrition oil to have the functioncompared to the VEGF group which the more obvious promotioninjured area heals.3. After the technique the 3 days blank group collagen fiber volumevalue is very low, the VEGF group and the blank group comparesp＜0.05, but the healing-wound nutrition oil group and the blankgroup compares p＜0.01, explained the healing-wound nutritionoil group the early time to be able to produce more collagen fiberafter the wound in order to speeds up the seal to wound; Afterthe technique 7 days the healing-wound nutrition oil group valuegrowth is quicker, but after 14 days the healing-wound nutritionoil group value have not statistics significance compared to othertwo groups, explained the healing-wound nutrition oil groupcollagen fiber to produce reduces speed thus has avoided thecollagen fiber excessively the proliferation, has avoided theexcessively many scars formation.4. After the wound 3 days starts, the injured area center blood vesselendothelial cell number starts to increase, 7 days achieves the peak after the wound, hereafter gradually drops, 14 days obviouslydrops after the wound, creates the nutrition oil line 7 days bloodcapillary cross section amount density comparison group tocompare has extremely significance difference (p＜0.01),explained 7 days is the blood capillary forms the peak.5. In the healing-wound nutrition oil group CDK4 and the CD31 havemost obvious expression, the VEGF group is next, the blank groupis weakest. In 3 days, 7 days and 14 days different time the threegroups have the obvious difference, P＜0.05, from the molecularlevel explained the healing-wound nutrition oil to be able topromote the blood vessel occurrence, the multiplication thus topromote the injured area to heal.6. VEGFmRNA expression after 3 days or 7 days in each group has theobvious difference, but after 14 days it is basic indiscriminationleaves. After 7 days the healing-wound nutrition oil group' s theVEGFmRNA expression is the peak. The table showed thehealing-wound nutrition oil group to have strongly adjusts theVEGFmRNA expression the function. After technique 14 days theVEGFmRNA expression quantity in each group is indiscrimination,showed in the granulation repair later period, the repair tendsto completing, each group of VEGFmRNA expression also tends tothe balance. Therefore may in the certificate big mouse skinregenerative process give the healing-wound nutrition oil firmlyto be able to promote in the granulation tissue the VEGFmRNAexpression.Conclusion1. The healing-wound nutrition oil mainly promotes the hyperplasyof granulation tissue capillaries in the early after the wound,secretes more collagen fibers in order to speeds up the injuredskin to heal up.2. The healing-wound nutrition oil through promoting theconcrescence of the injured area and the epithelization whichinhibit hyperplasy of the fibroblast and oversynthesis of thecollagen in the injured area repair later period reduces the scars.The mechanism may be①the healing-wound nutrition oil promoteshair follicle, sebaceous glands, angiocellular proliferationwhen the skin has pathology injury;②the healing-woundnutrition oil reduces the scars through supplying the nutritionto promote the injured area epithelization.
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