Expression And Significance Of 14-3-3 And NF-κB In Vulva Lichen Sclerosis And Vulva Squamous Cell Carcinoma

Objective: To explore the expression and clinical significance of 14-3-3 and NF-κB in vulva lichen sclerosis(VLS)and vulva squamous cell carcinoma(VSCC).Methods: Immunohistochemical staining was conducted to detect expression of 14-3-3and NF-κB in 25 cases of VSCC,40 cases of vulva lichen sclerosis and 15 cases of normal genital skin tissue,the clinical and pathological data of patients with VSCC were collected for analysis.Results: The expression of 14-3-3 in VLS was significantly lower than normal vulvar skin tissue and the difference was statistically significant(P<0.05).The expression of14-3-3 in VSCC was significantly higher than normal vulva skin tissue and the difference was statistically significant(P<0.05).14-3-3 expression in VSCC tissue of III-IV stage was significantly higher than that of stage I-II(P<0.05).There were no significant differences of 14-3-3 expression in age,clinical stage and tissue differentiation degree(P>0.05).The expression of NF-κB in VLS was significantly lower than normal vulva skin tissue(P<0.05).The expression of NF-κB in VSCC was significantly higher than normal vulva skin tissue(P<0.05).NF-κB expression in VSCC tissue of III-IV stage was significantly higher than that of stage I-II(P<0.05).The expression of NF-κB in cases with lymph node metastasis was higher than those without lymph node metastasis(P<0.05).The expression of NF-κB in the moderately differentiated VSCC was higher than those well differentiated VSCC(P<0.05).The Spearman correlation analysis showed that 14-3-3 and NF-κB expressions was positively correlated in vulva lichen sclerosis and vulva squamous cell carcinoma.Conclusion: The expression of 14-3-3 and NF-κB was associated with occurrence and development of VSCC.The low expression of 14-3-3 and NF-κB was associated with occurrence of VLS.