Effects Of Bactrian Camel Milk On Histology And Cell Apoptosis Of Liver And Kidney In Rats Modeled With Type 2 Diabetes Mellitus

To investigate the protective effects of Bactrian camel milk on liver and kidney in type 2 diabetes mellitus(T2DM) rats and provide a theoretical reference for the clinical treatment of diabetes and its complications with use of camel milk effective constituents. T2 DM rat model was established by feeding high-calorie forage and intraperitoneal injection of streptozotocin, then were randomly divided into the diabetes model group(DMC) and three treatment groups, including low concentration camel milk(LCM) group, high concentration camel milk(HCM) group and metformin hydrochloride(MTH) group, healthy rats were taken as control. Within 4 weeks treatment, monitoring the physiological and biochemical index of rats, then use the technology of H.E, immunohistochemistry, western blotting, RT-q PCR, discusses the different doses of camel milk treatment on the influence of histology and cell apoptosis of liver and kidney in T2 DM. The results showed that the body weight decreased in T2 DM groups, compared with DMC and MTH group, the body weight in LCM group was significantly decreased(P<0.01). Given glucose within 120 min, the blood glucose concentration in each groups were increased and then decreased. Compared with DMC group, the blood glucose concentration of 4 time points were decreased. Meanwhile, camel milk reduced fasting plasma glucose(FPG) and fasting insulin(FINS) levels, increase insulin sensitivity index(ISI). After camel milk treatment, partial cells of liver restored normal struture, fatty degeneration significantly reduced, and with no cavitation. Kidney tissue structure was complete and clear, with no obvious expansion of glomerular capillaries, renal capsule and tubular epithelial were normal. Immunohistochemistry and western blotting methods showed that camel milk inhibited Bax(Bcl2-associated X protein, Bax) and NF-kB(nuclear factor-kappa B, NF-kB) proteins, up-regulated Bcl-2(B cell lymphoma/lewkmia-2, Bcl-2) protein. Compared with MTH, camel milk inhibited Bax and NF-kB m RNA in liver tissue, but increased caspase-3(cysteinyl aspartate specific proteinase 3, caspase-3) m RNA, decreased Bcl-2 m RNA. In addition, camel milk decreased Bax and NF-kB mRNA in kidney tissue, up-regulated Bcl-2 m RNA, and caspase-3 m RNA in LCM and HCM group with high expression level need to be investigated futher. Camel milk can improve physiological and biochemical indexes of T2 DM, inhibit the expression of related apoptosis protein or gene in liver and kidney tissue, the results suggest that Bactrian camel milk has protective effect on the liver and kidney in T2 DM.