Studies On The Alkaloids Of Ervatamia Hainanensis And Lycopodium Japonicum

This research mainly investigated the alkaloids of Ervatamia hainanensis Tsiang and Lycopodium japonicum,two Traditional Chinese Medicine.And 35 compounds were purified and elucidated from these two plants,including 4 novel compounds.In this study,part of the isolated compounds have been carried on cytotoxic activity test.1.In this study,several modern chramogrophic seperations were applied to isolate and purify the indole alkaloids from Ervatamia hainanensis Tsiang's dried stem.The structures of purified compounds were elucidated by using NMR and MS analysis and comparison with known related compounds.There were 19 indole alkaloids,including 14 monomer alkaloids and five Bis-indole alkaloids,including three novel monomer alkaloids.Cytotoxic assay showed that compound 17?Deoxytubulosine?had significant inhabitation against the human lung adenocarcinoma cell line A549 by the MTT assay,with IC₅₀ values of 2.51±0.55 p.M.2.In this study,we isolate and purify the indole alkaloids from Lycopodium japonicum by means of several modern chramogrophic seperations.By conducting NMR analysis and comparison with known related compounds,we will be able to explain the structure of known compounds,while the structures of new alkaloid were elucidated on the basis of 2D NMR correlations as well as comparison with similar compounds.And 16 Lycopodium alkaloids were obtained,including one novel alkaloid.All isolated alkaloids were assayed for cytotoxic activity,but none of them showed significant inhabitation against the human lung adenocarcinoma cell line A549,HepG-2,HeLa,Smmc 7721 and SKOV-3 by the MTT assay,all IC₅₀ value were beyond 200 ?M.