Clinical Significance Of IL-17 And Lymphocyte Subsets In The Patients With Non-small Cell Lung Cancer

Objective: Serum and pleural effusion IL-17,blood and pleural effusion lymphocyte subsets(CD3+,CD4+,CD8+,CD4+/CD8+,CD16+CD56+)in patients with NSCLC were measured,their relationship with clinical staging and malignant pleural effusion were analyzed.Preliminary analyzed the correlation between IL-17 and lymphocyte subsets in patients with NSCLC,to provide reference clinical data for the monitoring of lung cancer and the possible mechanism of malignant pleural effusion.Methods: A total of 60 patients with diagnosed NSCLC admitted to Shaoyang Central Hospital of Hunan Province from Jan.2016 to Feb.2018 were enrolled,60 volunteers undergoing health examination over the same period were included as control.Examed each patients IL-17,CD3+,CD4+,CD8+,CD4+/CD8+,CD16+CD56+ expression level by flow cytometry method,observe the IL-17,T lymphocyte subsets and NK cells in patients with NSCLC clinical pathological features of expression,and analyzed of IL-17 and the correlation of T cell subsets and NK cells.Furthermore,subgroup analysis was performed on patients with NSCLC based on whether they were combined with pleural effusion,and the expression level of IL-17 in patients with NSCLC combined with malignant pleural effusion was compared.SPSS 20 statistical software was used for data analysis.Result:(1)The expression of IL-17 in serum of NSCLC patients was significantly higher than that of healthy controls,and there was a significant difference between them(P<0.01).(2)The expression of CD3+,CD4+,CD4+/CD8+,CD16+CD56+ in blood of NSCLC patients was significantly lower than that of healthy controls,and there was a significant difference between them(P<0.05).The expression of CD8+ in blood of NSCLC patients was lower than that of healthy controls,and there was no significant difference between them(p>0.05).(3)The expression of IL-17 in serum of NSCLC patients was related to the cancer cell degree of differentiation and clinical stage,the difference was significant(P < 0.01),but not related to sex,age and pathological type(p>0.05).(4)The expression of IL-17 in NSCLC with malignant pleural effusion was higher than that in serum(P < 0.01).(5)The expression level of CD3+,CD4+,CD4+/CD8+,CD16+CD56+in the blood of NSCLC patients was related to the clinical stage(P < 0.01),The expression of CD3+,CD4+ and CD16+ CD56+ in blood of patients with NSCLC was related to the degree of differentiation of tumor cells,and the difference was statistically significant(P<0.05),but not related to sex,age,pathological type(p > 0.05).(6)The expression level of CD3+,CD4+,CD4+/CD8+,CD16+CD56+in blood of patients with NSCLC complicated with malignant pleural effusion was similar to that in pleural effusion(P > 0.05).(7)There was no correlation between the expression of IL-17 in blood of NSCLC patients and the expression of CD3+,CD4+,CD4+/CD8+,CD16+CD56+(correlation coefficient r:0.247?0.089 ?0.094?0.085?0.164;Corresponding p value :0.058?0.499?0.474?0.520 ?0.211)Conclusions:(1)Serum IL-17 levels are increased whereas blood lymphocyte subsets and NK cell levels are decreased in patients with NSCLC,and are closely related to the clinicopathological features of NSCLC,preliminary analyzed the above indicators maybe provide reference clinical data for the monitoring of lung cancer(2)The expression level of IL-17 in pleural effusion of NSCLC patients with malignant pleural effusion was significantly higher than that in serum,these results suggest that IL-17 may be one of the factors contributing to the occurrence of pleural effusion in the formation of malignant pleural effusion.(3)The results of this experiment suggest that there was no correlation between IL-17 and T ymphocyte subsets?NK cell expression in NSCLC patients,it is needed further studied to know specific mechanisms.