The Establishment Of Quality Evaluation System Of Notopterygium

Objective:To improve the analytical method of TLC,in order to provide a more powerful auxiliary means for the overall quality evaluation of rhizome et radix notopterygii.High performance liquid chromatography and gas chromatography were used to establish the fingerprints of non-volatile and volatile components of rhizome et radix notopterygii,in order to provide a rational basis for the quality evaluation of the two main active components of scorpion,for laying the theoretical foundation of more effective and reasonable evaluation and quality control.Conduct pesticide residue testing on rhizome et radix notopterygii,acquaint with the conditions of pesticide residues in the country,and conduct a more comprehensive quality evaluation of rhizome et radix notopterygii.Methods:The TLC was identified by two expansion systems,benzene-ethyl acetate(4:1)and chloroform-methanol(8:2).By observing the fluorescent spots of notopterol,isoimperatorin and nodakenin,distinguish the originand the authenticity of rhizome et radix notopterygii.The DAD-HPLC method was used to establish HPLC fingerprinting of non-volatile components.HPLC method was used with acetonitrile-0.1% phosphoric acid as mobile phase,gradient elution,column temperature 25 ?,detection wavelength 310 nm;gas chromatography detection conditions,inlet temperature is 260 ?,detector temperature is 280 ° C,temperature is programmed,and volatile components are established.GC fingerprinting,and confirming each common peak by mass spectrometry;Gas chromatography-mass spectrometry and high performance liquid chromatography were used to evaluate the residual pesticide residues.Results:The results of TLC showed that the system(1)benzene-ethyl acetate(4:1)could detect the notopterol and isoimperatorin,and the system(2)chloroform-methanol(8: 2)could detect the nodakenin.The chromatographic behaviors of the Notopterygium inchum Ting ex H,T-Chang and Notopterygium franchetii H.de Boiss were consistent under the two systems.It can be seen from the fluorescence intensity of the spot that the content of notopterol in the Notopterygium inchum Ting ex H,T-Chang is generally higher than that in the Notopterygium franchetii H.de Boiss,and the content of isoimperatorin and nodakenin in Notopterygium franchetii H.de Boiss is higher than that in the Notopterygium franchetii H.de Boiss.The results of the established GC fingerprints showed that there were 10 common peaks in Notopterygium inchum Ting ex H,T-Chang,12 common peaks in Notopterygium franchetii H.de Boiss,inculding 9 identical constituents.The results of HPLC fingerprint showed that 9 common peaks in Notopterygium inchum Ting ex H,T-Chang,7 common peaks in Notopterygium franchetii H.de Boiss,inculding 6 identical constituents.Among them,the content of notopterol and isoimperatorin in Notopterygium inchum Ting ex H,T-Chang and Notopterygium franchetii H.de Boiss are significantly different.The results of GC and HPLC fingerprints showed that among the 222 batches,there were 7 batches of spurious breeds,21 batches of Notopterygium franchetii H.de Boiss samples,118 batches of Notopterygium inchum Ting ex H,T-Chang samples,and 76 batches containing both of them.The results were consistent.Pesticide residue test results showed that 65 of the 202 batches were detected for pesticide residues,such as Methoxychlor?Thiophanate-methyl?Phoxim?Dieldrin?Moncrotophos?Phorate.Conclusion:The TLC test is supplemented to provide a more powerful means for identifying the original and authenticity of the sputum.The established gas phase and liquid phase fingerprints are consistent with the original results of 222 batches of rhizome et radix notopterygii,indicating that the fingerprints established in this experiment can effectively distinguish the primitives and authenticity of the live mites,and can effectively characterize the quality of the medicinal materials.Provide a basis for overall quality evaluation and control.The QuEChERS-GC-MS spectrometry was used to detect the 27 pesticide residues in the rhizome et radix notopterygii.The detection of thiophanate-methyl and phoxim was established by HPLC.This method is quick and simple and can be used for simultaneous determination of methyl thiophanate and phoxim in samples.