Vascular Endothelial Growth Factor And Chemokine Receptor-3 In The Pathogenesis Of Pterygium

Background: Pterygium is a common ocular surface disease with high incidence.It is mainly a chronic process,which is characterized by the progressive growth of the conjunctival and subconjunctival fibrous vascular tissue to the corneal surface,which not only affects the appearance but also causes For visual impairment,the main treatment is surgical resection,but some patients are prone to recurrence after surgery.In order to fundamentally reduce its recurrence,research on its etiology and pathogenesis is crucial.The current research suggests that the disease is mainly a proliferative disease,and the formation of neovessels plays an important role in its occurrence and development.Therefore,the mechanism of neovascularization and the role of related factors in this disease have become the current research hotspots.More studies have confirmed that vascular endothelial growth factor(VEGF)and its receptor are highly expressed in pterygium compared with normal conjunctival tissue,and have a significant effect on the production and development of pterygium..Recent studies have found that Chemokine receptor 3(CCR3)is also an important pathway regulating neovascularization in a variety of tumors,ocular neovascular diseases such as corneal neovascularization(CRNV),choroidal neovascularization(CNV)plays an important role in diseases,but there is no relevant research in pterygium.Objective: To investigate the expression of vascular endothelial growth factor(VEGF)and chemokine receptor 3(CCR3)in pterygium.Methods: The expression of VEGF and CCR3 were detected in 18 patients with pterygium and were compared with the normal conjunctiva epithelium in other 14 cases.the histological difference between the two groups was compared by hematoxylin and eosin(HE)staining.The expression of VEGF and CCR3 in pterygium and normal conjunctival tissues were analyzed by immunofluorescence and immunohistochemical to analysis.The m RNA expression of VEGF and CCR3 were assessed by Real-time PCRResults: 1 Immunofluorescence and immunohistochemistry discovered that there were significant differences of expression of VEGF and CCR3 between pterygium and normal conjunctive tissue(P<0.05).2.The results of real-time PCR indicated that VEGF m RNA and CCR3 m RNA expressions were higher in pterygium than in nomal conjunctiva epithelium(P<0.05).3.There was a positive correlation between VEGF and CCR3 in the pathogenesis of pterygium(P<0.05)Conclusion: The increase of VEGF and CCR3 in pterygium suggesting that the two factors may be involved in the pathogenesis,and they may work together in the origin and development of pterygium.