Expression Of LGR5,?-catenin And E-cadherin In Gastric Cancer And Their Relationship With Clinicopathological Feature

Objective To investigate the expression of Lgr5,?-catenin and E-cadherin in gastric cancer tissues and adjacent normal mucosa and its relationship with clinicopathological factors,and to analyze the correlation between Lgr5,?-catenin and E-cadherin expression.It is expected to provide a basis for the diagnosis,treatment and prognosis of gastric cancer.Methods Standardized collection of 139 cases of gastric cancer diagnosed by the Department of Pathology of Inner Mongolia Medical University Hospital from January 2013 to January 2018 and 139 cases of normal gastric mucosal tissue 2 cm away from cancer tissue were included in this experiment.All cases of gastric cancer were confirmed by pathology as primary gastric cancer,and there was no history of adjuvant radiotherapy,chemotherapy and endocrine therapy before surgery,and pathological paraffin sections were made.Immunohistochemistry was used to detect the expression of LGR5,?-catenin and E-cadherin in gastric cancer tissues and adjacent normal mucosa tissues.The relationship between the expression of LGR5,?-catenin and E-cadherin was analyzed and the relationship between the three was analyzed.Statistical analysis was performed on the data using SPSS 13.0.Results 1.The positive expression rate of LGR5 protein,gastric cancer tissue(61.87%)was significantly higher than that of adjacent normal mucosa(28.06%)(P<0.05).LGR5 protein is mainly expressed in cytoplasm or cell membrane in gastric cancer tissues.The positive expression rate of ?-catenin protein was significantly higher than that of normal gastric mucosa(92.09%)(51.80%)(P<0.05).The expression of ?-catenin protein in gastric cancer cells is absent,or abnormal cytoplasmic and nuclear staining occurs.The positive expression rate of E-cadherin protein was significantly higher than that of normal gastric mucosa(94.96%)(53.96%)(P<0.05).E-cadherin protein in the gastric cancer tissue cell membrane coloration is discontinuous,point-like,intermittent.2.The positive expression rate of LGR5 protein is related to the degree of differentiation of gastric cancer,the presence or absence of lymphatic metastasis and TNM staging.The positive expression rate of LGR5 protein was significantly higher in the high-medium differentiation group(73.87%)than in the poorly differentiated group(48.48%)(P<0.05);in the lymph node metastasis group(75.71%),it was significantly higher than that in the no-lymph node metastasis group(47.83%).P<0.05);I+II stage gastric cancer(69.14%)was significantly higher than stage III+IV gastric cancer(51.72%)(P<0.05).However,it was not related to the patient's age,gender,tumor location,tumor size,depth of invasion and whether there was distant metastasis(P>0.05).3.The positive expression rate of ?-catenin protein is related to the degree of differentiation of gastric cancer,the depth of invasion,the presence or absence of lymphatic metastasis and TNM staging.The positive expression rate of ?-catenin protein was significantly higher in the high-medium differentiation group(73.97%)than in the poorly differentiated group(27.27%)(P<0.05);the uninvasive serosal layer group(63.64%)was significantly higher than the invasive serosa.The stratified group(31.37%)(P<0.05);the lymph node metastasis group(75.36%)was significantly higher than the lymph node metastasis group(28.57%)(P<0.05);the I+II stage gastric cancer(72.84%)was significantly higher than III +IV stage gastric cancer(22.41%)(P<0.05).However,it was not related to the patient's age,gender,tumor location,tumor size and whether there was distant metastasis(P>0.05).4.The positive expression rate of E-cadherin protein is related to the degree of differentiation of gastric cancer,depth of invasion,distant metastasis and TNM staging.The positive expression rate of E-cadherin protein was significantly higher in the high-medium differentiation group(79.45%)than in the low-differentiation group(25.76%)(P<0.05).The uninvasive serosal layer group(64.77%)was significantly higher than the invasive serosa.The group(35.29%)(P<0.05);the distant metastasis group(68.25%)was significantly higher than the distant metastasis group(42.11%)(P<0.05);the I+II stage gastric cancer(64.20%)was significantly higher.In stage III+IV gastric cancer(39.66%)(P<0.05).However,it was not related to the patient's age,gender,tumor location,tumor size and lymph node metastasis(P>0.05).5.Among the 139 cases of gastric cancer,26 cases of LGR5 protein and ?-catenin protein were positively expressed simultaneously,and 7 cases were negative at the same time.Spearman rank correlation analysis showed that LGR5 protein was negatively correlated with?-catenin protein expression(r =-0.550,P < 0.05).6.Among 139 cases of gastric cancer,39 cases of LGR5 protein and E-cadherin protein were positively expressed simultaneously,and 17 cases were negatively expressed at the same time.Spearman rank correlation analysis showed that the positive expression of LGR5 protein and E-cadherin protein was negatively correlated(r=-0.220,P < 0.05).7.Among 139 cases of gastric cancer,52 cases of E-cadherin protein and ?-catenin protein were positively expressed simultaneously,and 44 cases were negatively expressed at the same time.Spearman rank correlation analysis showed that E-cadherin protein was positively correlated with ?-catenin protein expression.(r = 0.380,P < 0.05).Conclusions 1.LGR5 protein is highly expressed in gastric cancer tissues and low in normal mucosa tissues,suggesting that LGR5 protein may be a marker of gastric cancer stem cells,which may be a useful biomarker for detecting early gastric cancer.2.?-catenin protein and E-cadherin protein are highly expressed in normal mucosa adjacent to the cancer,but low in gastric cancer tissues,suggesting that the decreased expression of ?-catenin and E-cadherin may be involved in the development of gastric cancer.3.The expression of LGR5 protein is related to the degree of differentiation of gastric cancer,the presence or absence of lymphatic metastasis and TNM staging,suggesting that LGR5 protein may play an important role in the development of gastric cancer.4.The expression of ?-catenin protein is related to the degree of differentiation,depth of invasion,lymphatic metastasis and TNM staging,suggesting that ?-catenin may play an important role in the development and metastasis of gastric cancer.5.The expression of E-cadherin protein is related to the degree of differentiation,depth of invasion,distant metastasis and TNM staging,suggesting that E-cadherin may play an important role in the development and metastasis of gastric cancer,and its expression may lead to gastric cancer cells.It is malignant and easy to metastasize.6.In gastric cancer tissues,the expression of LGR5 protein was negatively correlated with the expression of ?-catenin protein and E-cadherin protein,suggesting that the increase of cell membrane expression of LGR5 protein in gastric cancer may be regulated by ?-catenin protein and E-cadherin protein.This may have important implications for the malignant transformation and invasive growth of tumors.7.There is a positive correlation between ?-catenin protein and E-cadherin protein expression in gastric cancer tissues,suggesting that there may be some mechanism interaction between ?-catenin protein and E-cadherin protein in the development of gastric cancer.Combined detection of LGR5 protein,?-catenin protein and E-cadherin protein may have important implications for early clinical diagnosis and assessment of the invasion and metastasis potential of gastric cancer.