Antitumor Activity And Application Of Polysaccharide From Inonotus Obliquus

Cancer is one of the main causes of death in the world,in recent years,the search for more effective and safer methods for the treatment of tumors has become a hot spot for researchers and medical workers.As a fungus,Inonotus obliquus is most favored.It is widely used in product development in Eastern Europe,Japan,South Korea and other countries,however,China is still at the initial stage of its application,and the products developed by the Inonotus obliquus are basically not available in the market.In China's Heilongjiang and Jilin area collected Inonotus obliquus is mainly as raw materials sold to Russia,North Korea and South Korea and other countries,therefore,the domestic product development of Inonotus obliquus is imperative.In this paper,we studied the dose-effect relationship between the anti-tumor effect of polysaccharides of Inonotus obliquus and the influence of product processing conditions on the anti-tumor activity of polysaccharides of Inonotus obliquus,characterization of antitumor activity of Inonotus obliquus polysaccharide;On the basis of this,through single factor test,orthogonal test and response surface analysis,according to the research results of the quantitative relationship between the antitumor activities of polysaccharides from Inonotus obliquus,the formulation and production process of inonorus obliquus polysaccharide anti-tumor oral liquid and beverage were optimized,and the corresponding products were prepared.The results of the study show that:(1)According to the standard method,each mouse was implanted subcutaneously in the right axilla with 0.2 mL of sarcoma 180 cells at a concentration of 1 x 10~7 cells/mL,to study the effect about different concentrations of IOP on mouse solid tumor inhibitory which was implant sarcoma 180 cells by right forelimb armpit subcutaneous injection and dose-effect relationship.The results showed that:IOP1(25mg/kg),IOP2(50 mg/kg),IOP3(100 mg/kg),IOP4(200 mg/kg),IOP5(400mg/kg)had significant inhibitory effects on the growth of S180 sarcoma in mice.The inhibitory rates were 16.16%,24.79%,30.08%,36.18%and 48.78%,respectively.When the concentration between 25 mg/kg~400mg/kg,the correlation coefficient of the regression equation of the concentration-inhibition rate is 0.937.The results showed IOP had obvious antitumor action,there was a good linear relationship between the concentration and tumor inhibitory rate.(2)The polysaccharide of Inonotus obliquus were processed after high temperature(80 to 120 degrees),pH(4~8),Ultraviolet radiation(10s to 30s),electron beam irradiation(1kGy to 5 kGy),ionic strength(0.5 mol/L to 1.5 mol/L)after processing conditions of the above products,a total of 15 groups of S180 mice were given to the stomach,each group of mice was given the same dose of Inonotus obliquus polysaccharide 100 mg/kg.The results showed that:the tumor inhibition rates of IOP6,IOP7,IOP8,IOP9,IOP10,IOP11,IOP12,IOP13,IOP14,IOP15,IOP16,IOP17,IOP18,IOP19,IOP20 each groups were 30.57%,29.27%,28.46%,29.67%,30.89%,31.71%,33.33%,32.93%,32.11%,30.12%,31.22%,33.13%,30.56%,30.37%,29.72%,it showed that the treated polysaccharides of Inonotus obliquus still had a strong antitumor activity,and there was no significant difference compared with the antitumor activity of the untreated Inonotus obliquus polysaccharide group.(3)The best product formula of Inonotus obliquus antitumor oral liquid is:the amount of IOP was 70mg/10mL,the amount of xylitol added 8%,citric acid added0.1%,the addition of compound stabilizer was 0.05%.At this time the oral liquid is brown,the taste is mellow and the sweet and sour is moderate.(4)The best technology of clarification of Inonotus obliquus antitumor oral solution is as follows:the addition amount of ZCT1+1?natural clarifying agent B/A was 6%:3%,the temperature was 60?and the stirring speed was 200r/min.In this case,the clarification effect of oral liquid is the best,oral liquid polysaccharide retention rate and light transmittance were 90.05%and 92.62%.(5)The best product formula of Inonotus obliquus antitumor functional beverage is:the amount of IOP was 0.4%,the addition of jujube thick pulp was 4%,the amount of compound stabilizer was 0.05%,and the content of xylitol was 5%.At this time,the Inonotus obliquus antitumor functional beverage is brown,the color is uniform,with the special aroma of jujube and Inonotus obliquus,and the taste is delicate and the sweetness is moderate.