| Inonotus obliquus (Pers. : Fr.) Pilát belongs to the pore fungus branch brown to lie the hole bacteria. Inonotus obliquus is one of valuable and rare medical fungi. In Russian it was named Chaga. The Chaga have the very good curative effect on cancer, heart disease, diabetes, AIDS etc. Many researches about Inonotus obliquus were done in foreign countries.Inonotus obliquus that grows on living trunks of the mature birch, elm, alder or beech trees, the wild resource is confronted with drying up increasingly, so further research on establishing technical project of artificial cultivation is urgent to be done. The carbon source,nitrogen source and C/N suitable for, and microelements and vitamin needed for the submerged culture of Inonotus obliquus were studied in this article. The results were as follows: The optimum inorganic nitrogen source for mycelia growth was ammonium tartrate, the optimum organic nitrogen source was soybean power, the optimum carbon source was glucose, the optimum C/N was 20:1, Mn promoted the growth of mycelia, the optimum vitamin was VB1. Taking the dry weight of mycelia as major evaluation index, first to select the optimal liquid cultivation mode of Inonotus obliquus mycelium, the optimal mode is: glucose 20 g/L, soybean power 30 g/L, KH2PO4 2 g/L, MgSO4·7H2O 0.5 g/L, VB1 0.005 g/L. The mycelial yield reached 0.794 g/100 mL after 15 days' incubation at 26℃.The extraction rate was from polysaccharides content which was determined with Phenol-sulfuric method. L9(34) orthogonal experiment was conducted, with the extraction rate of polysaccharides as indicator, to optimize the hot water extraction technology for the polysaccharides of Inonotus obliquus. The optimal extracting condition of polysaccharide among manpower mycelium of Inonotus obliquus is that the ratio of solid material to water was 1:10, extraction temperature was 80℃, extraction time was 3 h. The content of total polysaccharide of Inonotus obliquus reached 4.04 %.The optimum extraction technology is stable, reasonable and feasible.Through setting up mouse S180 tumor-bearing models, we carried out the research on the anti-tumor functions of the polysaccharide of Inonotus obliquus. The incellular polysaccharide of Inonotus obliquus's tumor suppression rate was from 30.13 % to 41.07 %, which was related with the dosage present. The extracellular polysaccharide of Inonotus obliquus's tumor suppression rate was from 23.29 % to 36.73 %, which was related with the dosage present, too. Cyclophosphamide's tumor suppression rate was 52.83 %. They all have extremely obvious function on the suppression of the S180 tumor's activity.Between incellular polysaccharide and extracellular polysaccharide, incellular polysaccharide of Inonotus obliquus's tumor suppression function was better than extracellular polysaccharide.Comparing with the control group, both incellular polysaccharide and extracellular polysaccharide could obviously or extremely obviously enhanced the S180 tumor-bearing mouse's thymus index and the spleen index. The incellular polysaccharide of Inonotus obliquus's thymus index was from 3.36±0.17 to 3.84±0.51, the spleen index was from 7.64±0.63 to 7.97±1.02, which was related with the dosage present. The extracellular polysaccharide of Inonotus obliquus's thymus index was from 2.7±0.30 to 3.21±0.29, the spleen index was from 7.43±0.61 to 7.57±0.53. Cyclophosphamide's thymus index was 2.04±0.34, which doesn't obviously reduce comparing with the control group; The spleen index was 4.35±0.62, which extremely obvious reduced the S180 tumor-bearing mouse's spleen index. In vivo, the results show that the polysaccharide can increase the thymus index and spleen index of S180 tumor-bearing mice.
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