| Objective: To observe the effect of invigorating the spleen,benefiting the kidney and promoting blood circulation on improving the sperm motility and symptoms of the patients with asthenospermia and the rat model of asthenospermia,and to further explore the pathomorphological mechanism of invigorating the spleen,benefiting the kidney and promoting blood circulation on improving the sperm motility,so as to provide scientific basis for the treatment of asthenospermia by Invigorating the spleen,invigorating the kidney and promoting blood circulation method According to.Methods: 1 according to the inclusion criteria,90 cases(6 cases)of asthenospermia with deficiency of spleen and kidney and stagnation of blood stasis were included in the study.They were treated by the method of invigorating the spleen and kidney and activating blood circulation(Jujing Zhuyu Decoction).Before and after treatment,TCM syndrome score,semen samples were collected and sperm quality analysis were conducted.Two In this study,the sperm motility of epididymis was measured by computer-aided semen analysis in the model rats of asthenospermia.The pathological morphology of testis was observed by HE staining and the ultrastructure of mitochondria in the tail of sperm was observed by transmission electron microscope.The effects of the method of invigorating the spleen,invigorating the kidney and activating blood circulation on the spermatogenic environment of testis were confirmed and the improvement of sperm motility was revealed The effect mechanism of force.Result: 1 Clinical researchClinical effect of invigorating the spleen,benefiting the kidney and activating blood circulation on asthenospermia of spleen kidney deficiency with stasisAccording to the criteria of clinical efficacy evaluation,84 cases were effective,10 cases were cured(11.9%),36 cases were effective(42.9%),29 cases were improved(34.5%),9 cases were ineffective(10.7%).The total effective rate was 89.28%.After treatment,sperm motility(a,a + b)was significantly higher than before treatment(P.84 patients were mainly 21-40 years old,accounting for 79.8% of the total.The average age was 33.83 ± 6.725 years old.The minimum age was 23 years old and the maximum age was 47 years old,2 experimental study 2.1 effect of invigorating the spleen,benefiting the kidney and activating blood circulation on sperm motility of ratsCompared with the normal group,the total sperm viability of the model group decreased significantly 2.2 histopathological observation of testisAfter the establishment of the model,the testicular lesions in the model group were serious,and the average rank of testicular lesions was significantly different from that in the normal group(P< 0.05);after the treatment,the testicular lesions in each treatment group were improved in different degrees,among which the improvement in the high dose group and the middle dose group were more obvious(P<0.05),and the improvement in the low dose group was not significant(P>0.05).2.3 ultrastructural examination of spermatozoa in testis by electron microscopyIn the ultrastructure,the basement membrane was more regular,the space between the supporting cells and the basement membrane was compact,the mitochondria were slightly swollen,the ridges of mitochondria were slightly broken,some of them were vacuolated,but the whole was better than the model group.Conclusion:The method of invigorating the spleen,benefiting the kidney and activating blood circulation can effectively treat asthenospermia of spleen kidney deficiency with stasis.The mechanism may be to reduce the damage to testis,improve the spermatogenic environment of testis,and restore its spermatogenic function. |
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