Expression And Significance Of TXNIP In Pathological Scar

Background and purposeScars are a collective term for the appearance,morphology,and histopathology of normal skin tissue caused by various traumas,and is the result of body wound repair.In the process of wound healing,a variety of cells,cytokines,and extracellular matrix participate in this process.Imbalance between anabolic and catabolic metabolism can form pathological scars.Vascular endothelial growth factor(VEGF)plays an important role in the formation of pathological scars.The expression of VEGF in pathological scars is significantly higher than that of normal skin tissues,and the expression of vascular endothelial growth factor receptor 2(VEGFR2)is up-regulated by paracrine effects.Recent related studies have shown that TXNIP is closely related to vascular endothelial cell neovascularization.TXNIP plays an important role in VEGFR2 phosphorylation,endothelial cell(EC)survival and migration.Related research shows that,chronic inflammation promotes the occurrence and development of pathological scars,and TXNIP can enhance oxidative stress,inflammation and excessive collagen deposition through the TXNIP-NLRP3-caspase-1-IL-1β/IL-18 pathway to promote fibrosis in a variety of tissues.Thioredoxin interacting protein(TXNIP)is an endogenous inhibitory protein in the Trx system.It is widely distributed in the body and has a variety of biological functions.It can induce oxidative stress,regulate cell growth,promote inflammatory responses,and regulate mitochondrial function effects.The research on TXNIP is currently focused on diseases such as cancer,diabetes,myocardial reperfusion injury,and tissue fibrosis.The study of TXNIP expression in pathological scars has not been reported.The software performs data statistics to explore the relationship between the expression level of TXNIP protein in pathological scars and the influence of related factors.Research objectThis study collected surgical specimens of keloids,hypertrophic scars,and normal skin from the First Affiliated Hospital of Zhengzhou University from June 2019 to December 2019.All patients were diagnosed with a combination of related medical history,signs and postoperative pathology.Among them,24 were keloids,30 were hypertrophic scars,and 17 were normal skin.Tissue specimens were routinely paraffin sectioned.Research methodsThe scar tissue was scored using the Vancouver Scar Scale,and the experimental tissues were tested for the expression of TXNIP protein in all sections by immunohistochemistry(SP).Semi-quantitative results were used to determine immunohistochemical staining.SPSS 21.0 software was used.Count data was tested by χ2.The test level wasα=0.05.P<0.05 was statistically significant.The TXNIP protein was studied in keloids,hypertrophic scars,and normal.Whether there is a difference in the expression in the skin,the relationship between the expression of TXNIP protein and the clinical features of pathological scars(including:gender,age,location,accompanying symptoms,treatment history,family history)and the relationship with the level of VSS.Result1.The positive expression rate of TXNIP protein in keloid tissue was 83,33%,the positive expression rate in hypertrophic scar tissue was 66.67%,and the positive expression rate in normal skin was 17.65%.The positive rate of TXNIP protein in keloids and hypertrophic scars was significantly higher than that of normal skin tissues,and the difference was statistically significant(P<0.05),indicating that TXNIP protein was higher than that of normal skin tissues in pathological scars.2.In the study of the relationship between TXNIP and clinical characteristics of pathological scars,there were statistically significant differences in the effects of gender and concomitant symptoms on the positive rate of TXNIP expression(P<0.05).Age,location,treatment history,and family history affected TXNIP.There was no significant difference in the positive rate of protein expression(P>0.05).3.The positive expression rate of TXNIP protein was significantly different from the level of scar VSS score(P<0.05).Conclusion1.TXNIP protein is highly expressed in pathological scar.2.TXNIP protein expression was higher in women,patients with concomitant symptoms,and pathological scars with high VSS scores.3.TXNIP protein may promote the development of pathological scars by mediating angiogenesis and pro-inflammatory effects.