Preliminary Study On Quality And Hypoglycemic Mechanism Of Placenta Dry Powder Of Alashan Bactrian Camel

Objective:The research on camel placenta through inspection and collection found that the chemical composition and pharmacodynamics of camel placenta at home and abroad are not mature enough and not many.Mechanism of sugar action,explaining the mechanism of lowering blood sugar of camel placenta dry powder.Validate the use of camel placenta to treat diabetes through hypoglycemia.It will fill in the blanks of dry powder quality evaluation of Bactrian camel placenta and research on the mechanism of hypoglycemic effect.Methods:According to the Chinese Pharmacopoeia(2020edition),physical and chemical identification of Alashan Bactrian camel placenta dry powder(protein components and amino acid identification),general inspection(moisture,ash and extract content),thin layer identification(amino acid composition identification),The main component content determination(protein,trace elements and amino acid content),polymerase chain reaction identification,etc.for quality evaluation.A high-fat and high-sugar diet was combined with intraperitoneal injection of citric acid-sodium citrate buffered streptozotocin(STZ)to establish a rat model of hyperglycemia.Metformin hydrochloride tablets were used as positive controls.Gene and other levels,using a combination of traditional pharmacology and modern molecular biology,to detect fasting blood glucose,serum glycated hemoglobin(GHbALc),insulin(INS),glucose(GLUC3)content,and kidney GLUT2 and SGLT2 protein expressions And mRNA expression study the mechanism of Alashan Bactrian camel placenta dry powder on hypoglycemic rats.Results:(1)Physical and chemical identification results found that this medicinal material contains amino acid components and protein components.Alxa Bactrian camel placenta dry powder moisture content is 0.05%-0.08%,ash content 14,9%?15.0%,acid insoluble ash content 8.8%?9.0%,water soluble extract content 4.06%?5.65%,alcohol soluble extract content 2.65%?3.22%.Alxa Bactrian camel placenta dry powder heavy metal lead(Pb)content is 0.0588mg/kg,mercury(Hg)content is 1.41mg/kg,chromium(Cr)content is 57.28mg/kg,cadmium(Cd)content is 0.0002mg/kg,arsenic salt(As)content is 0.96mg/kg.Alxa Bactrian camel placenta dry powder TLC identification 70%ethanol decoction extraction,n-butanol:glacial acetic acid:water(5:1.5:1.5)to develop,0.2%ninhydrin ethanol color development,drying at 105 ? 3?5min The coloring spots of five amino acid components such as lysine hydrochloride,proline,threonine,tyrosine and alanine are separated and the separation degree is high.Alxa Bactrian camel placenta dry powder protein content is 15.05%?19.18%;trace element potassium(K)content is 0.6546%,calcium(Ca)content is 0.7672%,sodium(Na)content is 2.586%,magnesium The element(Mg)content is 0.3012%,the copper element(Cu)content is 0.0005%,the iron element(Fe)content is 0.4444%,the manganese element(Mn)content is 0.0060%,and the zinc element(Zn)content is 0.0055%;18 Aspartic acid(ASP)content is 5.155%,threonine(THR)content is 2.297%,serine(SER)content is 2.625%,glutamic acid(GLU)content is 6.676%,and glycine(GLY)The content is 6.815%,alanine(ALA)content is 4.025%,cystine(CYS)content is 0.883%,valine(VAL)content is 3.095%,methionine(MET)content is 0.868%,isoleucine Acid(ILE)content is 1.471%,Leucine(LEU)content is 4.611%,Tyrosine(TYR)content is 1.817%,Phenylalanine(PHE)content is 2.597%,and Lysine(LYS)content 3.653%,histidine(HIS)content is 1.663%,arginine(ARG)content is 3.990%,proline(PRO)content is 4.171%,tryptophan(TRP)content is 0.652%.The identification results of Alxa Bactrian camel placenta dry powder polymerase chain reaction proved that the medicinal material was camel placenta.(2)The mechanism of Alxa Bactrian camel placenta dry powder on hypoglycemia in hyperglycemic rats,referring to the preparation method and pre-experiment of diabetic hyperglycemic rat model,using high-fat high-sugar feeding combined with STZ modeling method Rats in other groups outside the group showed signs of sluggishness,polydipsia,polyuria,and yellowing of hair.After 3 days and 7 days of fasting blood glucose changes,the blood glucose value was? 17.88 mmol/L after 3 days of modeling and 7 days after modeling.The blood glucose value is? 17.49mmol/L.After modeling,there are"three more and one less”characteristics of polydipsia,polyphagia,polyuria and weight loss.Alxa Bactrian camel placenta dry powder has no significant changes in the weight,liver weight,kidney weight and pancreas weight of hyperglycemic rats,and it can reduce the fasting blood glucose.Alxa Bactrian camel placenta dry powder significantly reduced the serum GHbALc content in hyperglycemic rats,the serum INS content did not change significantly,and the serum GLUC3 decreased significantly.Alxa Bactrian camel placenta dry powder can down-regulate the levels of GLUT2 and SGLT2 protein GLUT2 and SGLT2 mRNA in kidneys of hyperglycemic rats.Conclusion:The results of Alxa Bactrian camel placenta dry powder quality research found that the experimental method had accurate results,good stability,reliable and complete methods,and could be used as the experimental reference for camel placenta.The results of the research on the mechanism of hypoglycemic effect of dry powder of Alxa Bactrian camel placenta have found that it can obviously reduce the content of fasting blood glucose,GHbALc and GLUC3 in the serum of hyperglycemic rats caused by the combination of STZ and high-fat and high-sugar feed.