Expression Of S100A6 In Patients With Adenomyosis

Background:Adenomyosis(AM)is a common and refractory benign disease in women of childbearing age,but it shows some biological behaviors similar to malignant tumors,such as abnormal proliferation of ectopic lesions,infiltration,and even invasion of adjacent tissue.The disease has many features,wide range,and extremely recurrent characteristics,which makes it difficult to be diagnosed early.More and more patients choose total hysterectomy because they cannot tolerate progressively worsening dysmenorrhea or cannot tolerate recurrence.It has caused great damage to the patient’s reproductive function,quality of life and even mental health.At present,the pathogenesis of AM and the mechanism of dysmenorrhea are not completely clear.Recent studies have revealed that S100A6 is likely to participate in the regulation of cell function and enhance the proliferation,migration and apoptosis of specific types of cells by changing cell differentiation and regulating signal transduction pathways to accelerate the progress of tumor cell cycle.Based on this,it is speculated that S100A6 may participate in the occurrence of adenomyosis and endometriosis by regulating the function of AM endometrial cells,promoting their infiltration and metastasis.Objective:This experiment aimed to study the expression level of S100A6 in related tissues and the serum expression level of CA125 of patients with adenomyosis,and explored its role in the occurrence and development of the disease and its relationship with dysmenorrhea in patients,in order to prove the endometrium of the patient can be obtained by endometrial biopsy and other methods,and theexpression of S100A6 in the endometrial tissue and the serum CA125 level can be combined to provide a theoretical basis for the diagnosis of adenomyosis.Methods:From August 2017 to February 2019,30 cases of adenomyosis with complete clinical data were selected as the case group,and 30 cases of hysterectomy due to hysteromyoma or CIN Ⅲ were selected as the control group.The degree of dysmenorrhea was evaluated by VAS before surgery.Immunohistochemical method was used to detect the expression intensity of S100A6 in the eutopic endometrium,ectopic endometrium of the case group and normal endometrium of the control group,and explore its relationship with the degree of dysmenorrhea.The serum CA125 was detected by electrochemical luminescence level,and explore its relationship with the degree of dysmenorrhea.SPSS 24.0 statistical software was used for statistical analysis.Measurement data using two independent samples t test.The eutopic endometrium and ectopic endometrium of the case group were compared with the eutopic endometrium of the control group,and two independent samples Wilcoxon rank sum test;Comparison of eutopic and ectopic endometrium in the case group,using two correlation sample rank sum test;The comparison of serum CA125 levels between the case group and the control group was performed using the Mann-Whitney U test;P<0.05 was considered statistically significant.The correlation between the expression level of S100A6 and the level of serum CA125 and the degree of dysmenorrhea in patients were analyzed by Spearman rank correlation test,correlation at P<0.05.The ROC curve analysis of S100A6,serum CA125 and the combination were performed to calculate the sensitivity and specificity of AM.Results:The expression level of S100A6 in the ectopic endometrium of the case group was higher than that of the control group,the difference was statisticallysignificant(P <0.05),and its expression level was positively correlated with the degree of dysmenorrhea(P <0.05).The expression level of S100A6 in the eutopic endometrium of the case group was higher than that of the control group,and the difference was statistically significant(P <0.05).The expression levels of S100A6 in the eutopic endometrium and ectopic endometrium of the same patient were similar,and the difference was not statistically significant(P> 0.05).The serum CA125 level was higher in the case group than in the control group,the difference was statistically significant(P <0.05),and the CA125 level was positively correlated with the dysmenorrhea of the patients(P<0.05).The sensitivity of S100A6,CA125 and the combined diagnosis of AM was 0.8,0.667 and 0.8,respectively.The specificity is 0.667,0.9 and 0.767.The AUCs are 0.761,0.797,and 0.822,respectively.Conclusion:1.The expression levels of S100A6 in the eutopic endometrium and ectopic endometrial tissue of the myometrium in patients with AM are higher than those in the control group,but the expression levels of S100A6 in eutopic and ectopic endometrium of the same patient are similar.2.There is a positive correlation between S100A6 expression level and dysmenorrhea in AM patients3.Serum CA125 levels in patients with AM are higher than those in the control group.4.There was a significant positive correlation between the level of CA125 and the degree of dysmenorrhea in AM patients.5.Detection of S100A6 and serum CA125 in uterine-related tissues has certain diagnostic value for AM;using endometrial biopsy method,combined detection of endometrial S100A6 and serum CA125 levels can improve the diagnostic compliance rate of AM.