Down-Regulation Of STAT3 And Fascin-1 Expression And The Effects Of Pituitary Tumor Cell Proliferation And Apoptosis

Object: To investigate the effect of down-regulating the expression of STAT3 and Fascin-1 on the proliferation and apoptosis of mouse pituitary adenoma cells.Methods: Mouse AtT20 pituitary adenoma cells were selected as experimental cells and divided into control group(PA group),STAT3 inhibitor vehicle group(PA + DMSO group),STAT3 inhibitor group(PA + BP-1-102 group),and Fascin-1 Negative control group(PA + neg-siRNA group),silent Fascin-1group(PA + Fascin-1-siRNA group).Using STAT3-specific inhibitor BP-1-102 to inhibit STAT3 protein expression in mouse pituitary tumor cells AtT20,at the same time,RNA interference(RNAi)technology was used to specifically inhibit the expression of Fascin-1 in mouse pituitary tumor cells AtT20.Immunoimmunofluorescence,Western blot,and Real-time RT-PCR were used to detect the expression of STAT3,Fascin-1,E-cadherin,and N-cadherin in the five groups,and CCK-8 and flow cytometry were used to detect the pituitary of the five groups of mice.Proliferation,apoptosis and cell cycle of tumor cells.The correlation between four histones and apoptosis and proliferation was detected by Pearson statistics.Results:(1)Immunofluorescence results: The expression of STAT3,Fascin-1,N-cadherin and PA +DMSO in the PA + BP-1-102 group was decreased,and the expression of E-cadherin was increased in the PA + BP-1-102 group.Compared with the PA + neg-siRNA group,the expression of STAT3,Fascin-1,and N-cadherin in the PA + Fascin-1-siRNA group was reduced,and the expression of E-cadherin was enhanced.(2)The expressions of STAT3,Fascin-1,E-cadherin and N-cadherin protein in five groups.The expression of STAT3,Fascin-1,N-cadherin in PA + BP-1-102 group was lower than that in PA + DMSO group the expression of E-cadherin was enhanced.Compared with the PA + neg-siRNA group,the expression of STAT3,Fascin-1,and N-cadherin in the PA + Fascin-1-siRNA group was reduced,and the expression of E-cadherin was enhanced.(3)CCK-8 detected the proliferation of AtT-20 cells: The proliferation ability of pituitary tumor cells in the PA + BP-1-102 group was decreased compared with the PA group.The proliferation ability of pituitary tumor cells in the PA + Fascin-1-siRNA group was decreased compared with the PA + neg-siRNA group.(4)Apoptosis and cell cycle results: The apoptosis rate of pituitary tumor cells in the PA + BP-1-102 group was increased compared with the PA + DMSO group,and the apoptosis of pituitary tumor cells in the PA + Fascin-1-siRNA group was increased.The rate was increased compared to the PA + neg-siRNA group.Compared with the PA + DMSO group,the PA +BP-1-102 group has a higher proportion of G1 phase cells and a lower G2 and S phase cell ratio;the PA +neg-siRNA group has a higher G1 phase than the PA + Fascin-1-siRNA group.The proportion of cells increased;the proportion of G2 and S cells decreased.(5)Statistical analysis shows that STAT3 and Fascin-1 are positively related to cell proliferation,STAT3 and Fascin-1 are negatively related to apoptosis,STAT3 is positively related to N-cadherin protein expression,and STAT3 and E-cadherin protein There was a negative correlation between expression,Fascin-1 and N-cadherin protein positive correlation,and Fascin-1 and E-cadherin protein negative correlation.Conclusions: STAT3 and fascin-1 are expressed in mouse pituitary tumors.Down-regulating the expression of STAT3 and Fascin-1 may be through up-regulating the expression of E-cadherin and down-regulating the expression of N-cadherin to inhibit the proliferation of pituitary tumor cells and induce apoptosis of pituitary tumor cells.