Effects Of Different Patterns Exercises On The Expression Of Related Proteins Of White Fat Browning In Growing Rats

Objective: Exercise as a healthy intervention can regulate energy balance by affecting the browning of white fat.However,the effects of different exercise methods on the browning of white fat are not the same,and the effect of exercise on the browning of white fat during the critical period of growth and development is not yet known.Therefore,this study intends to explore the effects of different exercise method on the expression of browning-related proteins of peripheral white adipose tissue and browning-related genes of hypothalamus in growing rats through animal experiments,and to explore the relationship between them.Methods: Male,3-week-old SD rats were divided into three groups,C group: Control group(sedentary,n=10);MD group: treadmill moderate-intensity exercise group(approximately 60% of VO2 max for 5 days a week for 4 weeks),HIIT group: High-intensity interval training(6 bouts of high-intensity treadmill running approximately 85%~90% of VO2 max exercise 6min,50% VO2 max exercise interval 4min,for 5 days a week,for 4 weeks).After the experiment,take the hypothalamus and the perirenal and epididymal white adipose tissue weights and of the rats are measured,use Real-Time-PCR to detect the expression level of POMC,NPY,LEPR and GRP78 m RNA of hypothalamus in each group;use Western Blot to detect expression level of PRDM16 and UCP1 protein of perirenal adipose tissue in each group.Results: 1.The weight of the three groups of rats increased continuously within 4 weeks of exercise intervention.Compared with group C,the weight of group E and group H increased slowly from the second week and there was a statistical difference(p<.05).2.The increase in absolute weight and relative weight of visceral fat in MD group and HIIT group was significantly lower than that in C group(p<.01);the increase in absolute weight and relative weight of visceral fat in MD group was significantly lower.In HIIT group,there was a statistical difference(p<.05).Compared with group C,the expression of PRDM16 protein in WAT of the kidneys in group MD was significantly increased(p<.01),and the UCP1 protein expression was significantly increased with statistical significance(p<.01).There was no significant difference in PRDM16 and UCP1 protein expression in WAT of HIIT group(p>.05).4.Compared with group C,the expression of NPY m RNA in hypothalamus in group MD was decreased,there was significant difference(p<.01),POMC,LEPR,GRP78 m RNA expression had no significant difference(p>.05);There was no significant difference in the expression of hypothalamus POMC,NPY,LEPR and GRP78 m RNA in HIIT group(p>.05).5.There was a negative correlation between the expression of NPYm RNA in hypothalamus and the expression of PRDM16 protein in white fat with statistical difference(p<.01).Conclusions: moderate-intensity exercise for 4 weeks in the growth and development period of rats can decrease NPY expression in hypothalamus of rats and induce white-to-brown phenomena in perirenal adipose tissue,but not in HIIT.