Effects Of Aerobic Exercise On Obese Mice Lipin1 Pre-mRNA Alternative Splicing

Objective: Observe the effects of aerobic exercise on mice body weight,blood lipids,adipose cell morphology and number.Investigate the the effects of aerobic exercise on Lipin1 pre-mRNA alternative splicing and splicing factor SFRS10.And explore the intrinsic relationship between aerobic exercise,Lipin1 pre-mRNA alternative splicing and splicing factor SFRS10.Methods: After 1 week feeding,forty C57BL/6 male mice were randomly divided into control group(C,n=20)and obesity group(O,n=20).After four weeks feeding,C group were divided into control group(C,n=10),control plus exercise group(CE,n=10),O group were divided into obesity group(O,n=10)and obesity plus exercise group(OE,n=10).Mice of the C group and CE group were fed with normal food,while mice in O group and OE group were fed with high fat diet.CE and OE group were on a treadmill exercise for eight weeks.We used the automatic biochemical analyzer to detect mouse blood lipids.And then we performed HE staining to observe lipid accumulation and adipocyte size.PCR and q PCR were performed to detect Lipin1 mRNA level,its isoforms' expression and splicing factor SFRS10 expression in mice liver tissue.Results:(1)After 4 weeks intervention,the body weight in O and OE group had significantly increased compared with the C group.After 8 weeks intervention,the weight in the CE group and OE group showed different degrees of decline,but the weight of mice in OE group was still higher than that in C group.After 12 weeks intervention,the weight in O group continuous rise and the weight in OE and CE group continuous decrease and lower than the C group.Both OE and CE group had a significant decrease compared with the C group.(2)In the O group,the expressions of TG,TC and LDL-C were high-regulated,and had a significant difference compared with the C group(P<0.05).The HDL-C expression was down-regulated in O group and had a significant difference compared with the C group(P<0.05).At the end of the 12 week,TG,TC,and LDL-C decreased and had the significant difference compared with the O group.The HDL-C level was considerably higher compared with O group(P <0.05).(3)Aerobic exercise affected the morphology and the number of adipocytes in obese mice.The O group had large morphology and small amount compared with the C group(p<0.05).After experience,the adipose cell in the CE group became aggregation and the number was markedly increased compared with the C group.Compared with the O group,the OE group had small morphology and increased number of fat cells(p<0.05).(4)The expression of Lipin1 and Lipin1? was high expressed in O group,and had the significant difference compared with the C group(P<0.05).After 8 weeks aerobic exercise,both Lipin1 and Lipin1? had a low expression in OE group and had the significant difference compared with the O group(p<0.05).Lipin1? was low expressed in the O group,and after the exercise,the level was increased considerably in both CE and OE group(p <0.05).(5)SFRS10 was low expressed in O group.After aerobic exercise intervention,there was a markedly increased in both CE and OE group(p <0.05).Conclusion:(1)Aerobic exercise can substantially reduce the body weight of obese mice,improve dyslipidemia,and change the adipocyte cells morphology and number in mice.(2)Aerobic exercise affects the expression of Lipin1 and regulates Lipin1 pre-mRNA alternative splicing,which changes the expression of its isoform: increases Lipin1? expression and decreases Lipin1? expression.Therefore,reduces the lipid generation and effectively reduces the incidence of obesity(3)Aerobic exercise can reduce the expression of obese related gene Lipin1 mRNA by increasing the concentration of splicing factor SFRS10,resulting in increased expression of Lipin1? and decreased expression of Lipin1?,thereby reducing the incidence of obesity.