Effects Of Polysaccharides From Dendrobium Candidum On Antioxidant Gene Expression In Rat Myocardium After Different Stress Training

According to the theory of free radicals,the main reason for aging is that the dynamic balance of oxidation and antioxidation is destroyed,which causes the accumulation of free radicals in the body and irreversible damage to the body,leading to oxidative damage and aging.However,sports training and fatigue damage directly lead to a large number of free radicals in the body.The balance between oxidation and antioxidation can be broken by different loads of exercise training.In order to maintain the balance between the generation and elimination of free radicals in the body in different loads of exercise training,we need to add some necessary antioxidants to the body to improve the activity of antioxidant enzymes in the body and enhance the ability of the organism to eliminate free radicals.In this experiment,we choose natural,green and efficient polysaccharide of Dendrobium officinale as antioxidant.The polysaccharide contained in Dendrobium officinale can enhance the activity of antioxidant enzymes,improve the ability of scavenging free radicals and antioxidation.Therefore,we boldly assume that taking Dendrobium officinale polysaccharide in sports training can reduce a large number of free radicals produced by sports training,maintain the balance of oxidation and oxidation resistance in the body,improve sports ability and prevent oxidative damage.Objective: To observe the gene level of Dendrobium candidum polysaccharide by intragastric administration of Dendrobium candidum extract in mice with different exercise load,The antioxidative effect on the body of mice under different sports load can be applied to the future sports training,sports rehabilitation and sports health care,and provide the athletes with green,healthy,safe and effective functional sports drinks,so that the athletes can speed up the recovery after sports,relieve fatigue,improve sports ability and extend sports life.Methods: 96 8-week-old male healthy mice with a body weight of(30 ± 2)g were selected from the animal experimental center of Shandong University of traditional Chinese medicine.According to 32 newly purchased mice in each group,they were randomly divided into 4 groups:(1)normal saline group,(2)polysaccharide group,(3)normal saline + exercise group,(4)polysaccharide +exercise group.According to the Chinese Pharmacopoeia,the daily consumption of Dendrobium candidum per person was recommended as 6-12 g,according to adult human body According to the calculation of 60 kg and the minimum dosage(6 g),the dosage of Dendrobium candidum should be 100.00 mg / kg · D and the trial conditions of 50,100,200,300 and 500 mg / kg were designed.The final intragastric dose of the experimental mice was determined to be 200 mg / kg.The mice were fed with adaptive feeding for one week,and then the mice were formally given swimming training for six weeks.The training time was 30 minutes in the first week,60 minutes in the second week,90 minutes in the third week,120 minutes in the fourth week,150 minutes in the fifth week and 180 minutes in the sixth week.At the second,fourth and sixth weeks,8 mice in each group were randomly selected to take out the complete myocardium and blood for preservation.The relative expression of SOD and GSH PX in myocardium was determined by RT-q PCR,and the content of MDA in serum was determined by colorimetry.Results: SOD m RNA: the sod m RNA of polysaccharide group was always higher than that of normal saline group,but there was significant difference only in the sixth week(P < 0.05);the sod m RNA of polysaccharide exercise group was always higher than that of normal saline exercise group,and there was significant difference in the second week(P < 0.05),the fourth and sixth weeks(P < 0.01);the sod m RNA of polysaccharide exercise group was always higher than that of polysaccharide group,and there was significant difference Difference,P < 0.01.Gsh-pxmrna: there was no significant difference between the two groups(P >0.05);the gsh-pxmrna in the polysaccharide exercise group was slightly higher than that in the saline exercise group,with significant difference only in the fourth week(P< 0.01);the gsh-pxmrna in the polysaccharide exercise group was higher in the second and fourth weeks than that in the polysaccharide group,with significant difference in the fourth week(P < 0.01)There was no significant difference(P >0.05).MDA: the MDA content of the normal saline group was always higher than that of the polysaccharide group,with a significant difference at the fourth week(P < 0.05),and at the sixth week(P < 0.01);the MDA content of the normal saline exercise group was always higher than that of the polysaccharide exercise group,with a significant difference at the fourth and sixth weeks(P < 0.05);the MDA content of the polysaccharide group was always higher than that of the polysaccharide exercise group,and at the fourth and sixth weeks(P < 0.05)There was significant difference(P< 0.05).Conclusion: Dendrobium candidum polysaccharide has a good antioxidant effect,and can improve the expression of GSH PX m RNA and SOD m RNA in the myocardium of exercise mice(1)The relative expression of GSH PX and SOD in myocardium was increased when the body with moderate exercise intensity took Dendrobium candidum polysaccharide;(2)The administration of Dendrobium candidum polysaccharide in the body with high exercise intensity can slow down the decrease of SOD relative expression level in myocardium,and has no effect on GSH px.The expression of antioxidant enzyme gene was affected,which further affected the content of antioxidant enzyme in vivo,increased the content of SOD and GSH PX in mice serum,and decreased the content of MDA in mice serum.