Research On The Self-assembly Properties And Application Of Chitosan Nanomaterials

Biocompatibility nanomaterials are payed more and more attention in the field of the healthcaremonitor biosensor.It well known that chitosan have been applied widely in drug delivery,tissue engineer,biosensor and medical device.In presence,most of the chitosan nanoparticles and nanofiber were prepared by expensive equipment and through complex method,which limited its application.In thesis,chitosan nanostructure were prepared by evaporation method,including chitosan-tartaric and chitosan-tartaric sodium.Using chitosan nanostucture as template by hydrothermal method,chitosan/CuO nanoparticles and hydrothermal carbonization/Cu₂O?HTC/Cu₂O?nanofibers were synthesized.Chitosan/CuO nanoparticles and HTC/Cu₂O nanofibers were in site on the surface of carbon cloth to form the chitosan/CuO carbon cloth electrode?chitosan/CuO-CF?and HTC/CuO carbon cloth electrode?HTC/CuO-CF?,respectively.The chitosan/CuO-CF and HTC/CuO-CF showed good electrochemical catalyze glucose properties.According the flexible of carbon cloth,chitosan/CuO-CF and HTC/CuO-CF would be a kind of non-enzyme glucose biosensor which were applied in the wearable microelectrode device.The main innovations of the thesis as follow:?1)Chiosan-tartaric and chitosan-tartaric sodium were prepared by evaporation method which was simple method without complex pure method.2)The alcohol disperse of chitosan-tartaric could be stable for 7-30 day.3)Chitosan-tartaric sodium were self-assembled to multidomain fiber network,nanorod and nanosheet.4)Chitosan/CuO-CF and HTC/CuO-CF have good electrochemical catalyzed glucose properties,and the limitdetection concentration were 54.7nM and59.1nM.5)Aginside chitosan/Ag membrane was gradually moved to surface of membrane to form nanoparticles,nanoplate,nanocube and nanorod tree.The main contents and conclusion of the thesis as follow:?Chitosan nanomaterials were prepared by evaporation method using different pure method,including chtosan-tartaric?CS-TA?and chitosan-tartaric sodium?CS-TA?.The structure of chitosan nanomaterials were characterized by FTIR,DSC,XRD.The morphologies and the properties of chitosan nano materials were characterized by POM,SEM and TEM.The results shown that CS-TA was dispersed in ethanol and ethanol solution to form nanogel with 500nm diameter,and the dispersed solution could stable from 7day to 30day.CS-TA could be self-assembled to multilayer nanosheet with 200-300nm thickness and 50?m length,and nanorod crystal with10?m diameter and more than 100?m length.CS-TA-Na power could be dispersed in solution?pH=7-8?to form nanofiber dispersed solution,in which nanofiber was 20-30nm diameter and 100-200nm length.CS-TA-Na could be self-assembled to form nanofiber and the multidomain nanofiber network on roughness and the polarity FTO substrate.CS-TA-Na was self-assembled to form the nanorod and nanofiber on the glass and polytetrafluorothylene?PETF?substrate under 4?,respectively.CS-TA-Na nanorod and nanosheet vertical distribution substrate were formed at 60?.The nanorod were 100nm diameter and300-500nm length,nanosheet with 20-30nm thickness.The multidomain nanofiber network were growth with the nanofiber with 20-30nm diameter and 100-200nm length.The machine of CS-TA-Na self-assembly was accorded the nucleation and fiber network formation growth.Such controllable morphologies of nanorod,nanosheet and nanofiber might be served as the templates for subsequent biomineralization or biosensor.Chitosan/CuO was synthesized at 90? and 120? by hydrothermal method using chitosan-tartaric nano gel as template.A novel chitosan/CuO carbon cloth electrode?CuO/CS-CF?were prepared by chitosan/CuO in site on carbon cloth.The structure and morphologies of chitosan/CuO nanocomposite were characterization by FTIR,XPS,XRD,SEM and TEM.The electrochemical glucose sensor of HTC/Cu₂O-CF were characterized by amperometric and potentiometric methods.There existed pompon morphologies of chitosan/CuO preparation at 90? temperature with 250-300nm diameter which was composed of nanoparticles with 10nm diameter.The morphologies of chitosan/CuO synthesized at 120? temperature was pompon with 1?m diameter which was aggregated of nanowisker with 10nm diameter and50nm length.The chitosan/CuO nanocomposite could be dispersed in water to form ink which was stable more than 7days at room temperature.There existed sea urchin hollow morphologies of chitosan/CuO on carbon cloth which aggregated of nanowisker with 10nm diameter and 50nm length.CuO/CS-CF sensor for glucose oxidation displays high sensitivity(15014.11?A cm^-2 mM^-1)with a high detection limit?LOD of 54.7nM?,short response time?within 6s?.These results clearly indicate that the flexible CuO/CS-CF might be a kind of glucose sensor applied in the biological field in the future.HTC/Cu₂O was synthesized at 180? hydrothermal method by mixture of chitosan-tartaric nanogel and Cu?COOH?₂.HTC/Cu₂O carbon cloth electrode?HTC/Cu₂O-CF?were prepared by HTC/Cu₂O in site on carbon cloth.The structure and morphologies of HTC/Cu₂O were characterization by FTIR,XPS,XRD,SEM and TEM.The electrochemical glucose sensor of HTC/Cu₂O-CF were characterized by amperometric and potentiometric methods using electrochemical work station.The results showed that HTC/Cu₂O nanoparticles could be dispersed in water to form ink which was stable more than 7 days at room temperature.HTC/Cu₂O nanofiber with20-50nm diameter and more than 500?m length.HTC/Cu₂O-CF sensor for glucose oxidation displays high sensitivity(3287.9?A cm^-22 mM^-1)with a high detection limit?LOD of 59.1nM?,short response time?within 6s?.The HTC/Cu₂O-CF nanofiber were prepared by simple method suggested the chitosan-tartaric nanogels was a kind of method for 2 D nanofibers.Chitosan/silver nanocomposite?CS/Ag?were synthesized using chitosan-tartaric sodium as the template.CS/Ag membrane was prepared by casting film.The structure and morphologies of CS/Ag nanocomposite were characterization by FTIR,XRD,SEM and Uv-vis.The results shown that Ag nanoparticles inside CS/Ag membrane showed good stability in air environment.When CS/Ag membrane was store in air for6-240h,Ag nanoparticles was gradually moved from inside to surface of CS/Ag membrane.There existed nanoparticles with 10-150nm diameter and its aggregated,nanoplate with 20nm thickness and 100nm width and length,nanocube with 150-250nm.The novel CS/Ag membrane maybe a kind of long antibacterial materials by Ag gradually release.