| Purpose:In this experiment,the effect and mechanism of anti nerve cell apoptosis after cerebral ischemia injury of kidney brain compound soup combined with NSCs transplantation were investigated by observing the symptoms of nerve function loss in MCAO rats,the area of cerebral infarction and the expression of HSP70,AIF protein,and the non casepase dependent apoptosis pathway mediated by HSP70 and AIF.Methods:180 adult SD rats were randomly divided into sham operation group,model group,Shennao Fuyuan decoction group and Shennao Fuyuan decoction combined with NSCs transplantation group.The neurological impairment scores were evaluated before and 1 day after treatment,and the animals were killed at the corresponding time points.The volume of cerebral infarction was detected by TTC staining,the expression of HSP70 and AIF was detected by immunohistochemistry and Western blot method.Results:1.Neurological deficit score:After treatment for 1 d or 3 d,the m-NSS scores of the traditional Chinese medicine group and the combined group were significantly lower than those of the model group at the same time point(P<0.05 or P<0.01).Among them,the decrease was more obvious in the combined group,the difference between the two groups was statistically significant(P<0.05 or P<0.01).2.Computation of cerebral infarction volume:After 1 d or 3 d of treatment,the cerebral infarct size in the traditional Chinese medicine group and the combined group decreased compared with the model group at the same time point,the difference between the two groups was statistically significant(P<0.01 or P<0.05).Among them,the degree of decrease was more in the combined group,the difference was statistically significant(P<0.05).3.Immunohistochemical detection of HSP70 protein expression:The expression of HSP70 in model group,traditional Chinese medicine group and combined group reached the peak about 3 days after operation,then decreased gradually.The expression of HSP70 in the Chinese medicine group and the combined group was significantly higher than that in the model group at the same time point after treatment for 1 d or 3d,and there was significant difference between the two groups(P<0.01).Among them,the increase was more obvious in the combined group,and the difference between the two groups was statistically significant(P<0.01).4.Expression of AIF protein was detected by immunohistochemistry:The expression of AIF protein in model group,traditional Chinese medicine group and combined group reached the peak about 3 days after operation,and then decreased gradually.The expression of AIF protein in the Chinese medicine group and the combined group was significantly lower than that in the model group at the same time(P<0.01),and the expression of AIF protein in the combination group was significantly lower than that in the model group at the same time point(P<0.01).Among them,the decrease was more obvious in the combined group,and the difference between the two groups was statistically significant(P<0.01).5.Western blot method for detecting the expression of HSP70 protein:The expression of HSP70 protein in model group,traditional Chinese medicine group and combined group reached the peak about 3 days after operation,and then decreased gradually.The expression of HSP70 protein in the traditional Chinese medicine group and the combined group was significantly higher than that in the model group at the same time point,and the difference between the two groups was statistically significant(P<0.01).The difference between the two groups was statistically significant(P<0.05 or P<0.01).6.Western blot method for detecting the expression of AIF protein:The expression of AIF protein in model group,traditional Chinese medicine group and combined group reached the peak about 3 days after operation,and then decreased gradually.The expression of AIF protein in the traditional Chinese medicine group and the combined group was significantly lower than that in the model group at the same time point(P<0.01),and the difference between the two groups was significant(P<0.01).Among them,the decrease was more obvious in the combined group,and the difference between the two groups was statistically significant(P<0.01).Conclusion:1.Both Shennao Fuyuan decoction and Shennao Fuyuan decoction combined with NSCs transplantation can increase the anti-stress ability of the body by regulating the cellular stress mediated by HSP70 and the non-casepase dependent apoptosis pathway mediated by AIF.It can protect the ischemic brain cells and promote the recovery of nerve function.2.The neuroprotective effect of Shennao Fuyuan decoction combined with NSCs transplantation on ischemic brain cells is better than Shennao Fuyuan decoction. |
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