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Effect of extraction conditions and oxidative crosslinking on the chemical structure and microbial fermentability of arabinoxylans

Posted on:2014-08-31Degree:M.SType:Thesis
University:University of MinnesotaCandidate:McClatchey, Bridget ColleenFull Text:PDF
GTID:2451390005484454Subject:Agriculture
Abstract/Summary:
The aim of this thesis project was to obtain corn bran AX of varying structure by using different extraction conditions, oxidatively cross-link them, and determine the microbial fermentability of both the non-cross-linked and cross-linked samples.;To reach this goal the following objectives were defined: *Determine the influence of NaOH concentration used for AX extraction on structural characteristics of extracted AX. *Determine the influence of a post extraction procedure, ball milling, on the structural characteristics of extracted AX. *Cross-link differently extracted AX by using laccase and oxygen. *Determine the effects of ferulate cross-linking on fermentability in an in vitro fermentation experiment and analyze fermentation end products (pH, gas volume, and SCFAs). *Determine the release/formation of phenolic metabolites by gut microorganisms as well as the percentage of original FA being metabolized by gut microbiota.
Keywords/Search Tags:Extraction, Extracted AX, Fermentability
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