Effects Of Anaerobic Treatment On Postharvest Physiology And Quality Of Agaricus Bisporus

Agaricus bisporus is not only delicious,but also rich in nutritional value,and has certain health benefits.It has the functions of inhibiting tumor development,lowering blood pressure,improving nerve function and lowering blood lipids.Agaricus bisporus is the edible fungus with the widest cultivation range,the largest planting scale and the largest production quantity in the world.The production and consumption in China are increasing year by year.However,due to the strong post-harvest respiration of Agaricus bisporus,browning is prone to occur.The shelf life at room temperature is only 1-3 days,which restricts the circulation and sales of Agaricus bisporus.This article studies the effects of high concentration of anaerobic inert gas(carbon dioxide/nitrogen)on post-harvest physiology and quality of Agaricus bisporus,in order to provide a certain theoretical basis and technical support for post-harvest storage of Agaricus bisporus.1.This paper conducted a study on single treatment and mixed treatment.The single treatment includes spreading treatment(CK),high carbon dioxide treatment(T1),high nitrogen treatment group(T2),and spontaneous modified atmosphere group(T3).Samples were taken at 0 h,6 h,24 h,72 h,168 h,and 336 h.The mixed treatment includes high carbon dioxide treatment + spontaneous modified atmosphere treatment(T21)and high nitrogen treatment + spontaneous modified atmosphere treatment(T22),which are carbon dioxide/nitrogen treatment 72 h + spontaneous modified atmosphere treatment 96 h and carbon dioxide/nitrogen treatment 72 Samples were taken after h+ spontaneous modified atmosphere treatment for 264 h.Detection of browning index,p H value,soluble protein,amino acids,ascorbic acid,malondialdehyde and other indicators of Agaricus bisporus,as well as polyphenol oxidase(PPO),peroxidase(POD),catalase(CAT)and other enzymes live.The results show that:(1)The anaerobic treatment of Agaricus bisporus can maintain a good color when stored for 336 h,which suppresses the browning and the reduction of soluble protein of Agaricus bisporus,and the result of single treatment is better than mixed treatment;(2)Anaerobic treatment can increase the amino acid content of Agaricus bisporus.When stored for 24 hours,the total amino acid content of T1,T2,CK is 32.73mg/g,63.75mg/g,23.33mg/g;(3)Anaerobic Treatment can effectively inhibit the decline of ascorbic acid and single treatment has better positive effects than mixed treatment;(4)Anaerobic treatment significantly inhibits the increase ofmalondialdehyde content and PPO activity,and the malondialdehyde content of CK-treated Agaricus bisporus is in The content increased sharply at the end of storage,which was 2.02 times of T1 and 2.73 times of T2;mixed treatment can better suppress the rise of malondialdehyde content than single treatment;(5)in PPO and CAT enzyme activity measurement,Oxygen-treated Agaricus bisporus better inhibits the decrease of PPO and CAT activity than natural spreading treatment,while T21 better inhibits the increase of PPO activity and the decrease of CAT activity than T1,and T22 is the opposite result compared with T2.2.The changes of polyamine and related enzyme activity of Agaricus bisporus under high concentration carbon dioxide treatment and high nitrogen treatment were studied.The results showed that: under natural conditions,the polyammonia content in Agaricus bisporus increased sharply,but the high concentration of carbon dioxide treatment and nitrogen treatment significantly inhibited the growth of polyamine content.The detection of key enzyme activities in the polyamine metabolism pathway revealed that anaerobic treatment did not Will hinder the activity of polyamine synthase in Agaricus bisporus.Instead,it promotes the decomposition of polyamines and generates a large amount of ?-aminobutyric acid(GABA)to transfer the nitrogen flow in the body.Therefore,adopting appropriate anaerobic fresh-keeping treatment can not only prevent Agaricus bisporus from spoiling and spoiling,but also effectively improve its nutritional quality composition,which has the effect of improving quality and keeping fresh.In summary,high-concentration anaerobic treatment has a significant effect on maintaining the quality and anti-oxidation ability of Agaricus bisporus during post-harvest storage,and has great application potential.