Study On The Co-fermentation Of Ganoderma Lucidum And Saccharomyces Boulardii And Its Effect On Polysaccharide Yield And Activity

Ganoderma polysaccharide is an active substance produced in the growth process of Ganoderma lucidum.Its pharmacological activity has been widely recognized and recognized.With the continuous in-depth study,probiotic fermentation technology has become increasingly mature.In order to improve the yield of ganoderma polysaccharide,this paper mainly uses the method of liquid CO fermentation of ganoderma mycelium and bladder's yeast in probiotics.In addition,the in vitro antioxidant activities of glep 1 produced by CO fermentation and glep 2 produced by traditional liquid fermentation were compared.The results are as follows:(1)According to the growth regulation of Ganoderma lucidum mycelium and Saccharomyces boulardii.Liquid fermentation and the change of substances in the fermentation process,explore the inoculum amount,inoculation time and co-fermentation time of Saccharomyces boulardii.Fermentation broth during co-fermentation.The experimental results show that the most suitable fermentation process is:at 10%inoculation volume,insert Saccharomyces boulardii fermentation broth(fermentation broth OD value is 1.0)at the 48th hour when the Ganoderma lucidum mycelium enters the fermentation stage,and then carry out A total of 72h fermentation.Under the conditions of this fermentation process,the yield of Ganoderma lucidum polysaccharide is increased by nearly 102%compared with the traditional pure Ganoderma lucidum liquid submerged fermentation.(2)The fermentation process of co-fermentation of Ganoderma lucidum mycelium and Saccharomyces boulardii to produce extracellular polysaccharide was optimized by single factor test and response surface test.The experimental results show that the optimal co-fermentation culture ratio is:maltose 32.0 g/L,yeast powder 13.5 g/L,initial pH is 5.7,which can meet the highest production of Ganoderma lucidum mycelium and Saccharomyces boulardii.Ganoderma lucidum polysaccharide production.(3)In vitro antioxidant analysis of glep 1 and glep 2 was carried out by ABTS free radical scavenging method,DPPH free radical scavenging method,hydroxyl radical scavenging method and reduction force measurement.The results showed that glep 1 and glep 2 showed certain antioxidant properties in different antioxidant methods and the antioxidant properties were the same.The results showed that the in vitro antioxidant activity of exopolysaccharides produced by CO fermentation did not change.