Effects Of Different Shading On Leaf Color And Anthocyanin Content Of Red And Green Ardisia Mamillata Hance Cutting Seedlings

Ardisia mamillata Hance is a cherished plant of the genus Ardisia Swartz in the family Myrsinaceae.Found in China in the 1970 s,it has high ornamental value and certain medicinal value,but at present it has not been popularized.In this study,we usedthe cutting seedlings of red and green Ardisia mamillata Hance as test materials.Four different gradient shade groups were set up,respectively 0%,25%,50% and 75%.Under these different shade conditions,two varieties of Ardisia mamillata Hance were systematically studied.We have explored their variation of leaf color,changes in the synthesis and accumulation of anthocyanin,and also their simple physiological response characteristics,in order to further understand the differences in leaf colors,physiological characteristics.It can also provid the theoretical basis and data support in Southwest region selection,new Variety cultivation and garden configuration.The main research results are as follows:(1)As far as photosynthetic pigments are concerned,Shading increased the contents of chlorophyll a,chlorophyll b,and carotenoids in the leaves of red and green Ardisia mamillata Hance.The decline of photosynthetic pigments in the initial experimental group indicates that Ardisia mamillata Hance may have a weak adaptability to light changes,but the shade environment It is more beneficial to increase the total amount of photosynthetic pigments of two kinds of Ardisia mamillata Hance,so that it can improve its own photosynthetic efficiency,and it can also accumulate photosynthetic pigments and deepen the color of the leaves.The chlorophyll a/b value of both Ardisia mamillata Hance is less than 3,but the chlorophyll a/b value of red Ardisia mamillata Hance will show an upward trend and will approach 3,indicating that red Ardisia mamillata Hance can better adapt to shade condition,and make full use of diffuse light。(2)The activity of phenylalanine ammonia lyase,a key enzyme in anthocyanin synthesis,was significantly positively correlated with anthocyanin in red Ardisia mamillata Hance,but not in green Ardisia mamillata Hance.Similarly,the total amount of chlorophyll is also positively correlated with anthocyanin in red Ardisia mamillata Hance,but not significant in green Ardisia mamillata Hance.18 kinds of anthocyanins contained in Ardisia mamillata Hance were determined by liquid chromatography-mass spectrometry technique,among which red Ardisia mamillata Hance was mainly composed of cornflower and green Ardisia mamillata Hance was mainly composed of mallow pigment;by comparing two kinds of Ardisia mamillata Hance,shading can effectively increase the abundance of anthocyanin species in the leaves of Ardisia mamillata Hance.By comparing the changes of differential metabolites of Ardisia mamillata Hance in the anthocyanin metabolism pathway,although shading can increase the abundance of anthocyanin species in the leaves of Ardisia mamillata Hance,it does not accumulate anthocyanins quickly and effectively,especially for green Ardisia mamillata Hance,the effect is very small,and there is a big difference in the accumulation of anthocyanins between the two Ardisia mamillata Hance,shading can only deepen the leaf color of the two Ardisia mamillata Hance to a certain extent,and can not change the anthocyanin metabolism process of two kinds of Ardisia mamillata Hance.(3)From the physiological response to light stress,the soluble sugar of red Ardisia mamillata Hance has been maintained at a low level,while the green Ardisia mamillata Hance has maintained a high level,this indicates that red Ardisia mamillata Hance is more adaptable to shade environment than green Ardisia mamillata Hance,and higher soluble sugar content does not make green Ardisia mamillata Hance accumulate anthocyanins faster.The soluble protein content of red and green Ardisia mamillata Hance reached its maximum at 40% shading and 50% shading after 40 days of shading,which may be because the plants increased the laser pigment protein to resist low light to improve its laser power and light energy utilization efficiency.Correlation analysis found that the soluble sugar and soluble protein of the green Ardisia mamillata Hance showed a very significant positive correlation.Combined with the shading conditions,it can be seen that the red Ardisia mamillata Hance is more suitable for the shady and weak light environment,and the green Ardisia mamillata Hance is more suitable for the light environment.Taken together,although the difference in red leaf color of the two Ardisia mamillata Hance is the result of artificial cultivation and natural differentiation,there are certain differences in various physiological aspects of the two Ardisia mamillata Hance.The red Ardisia mamillata Hance is more suitable for higher shades,the green Ardisia mamillata Hance is more adapted to survive under strong light.And the anthocyanin composition and relative content in the leaves of two kinds of Ardisia mamillata Hance are also quite different,the comparison of the different metabolites after shading shows that there are also great differences in the final products of anthocyanin metabolism of the two Ardisia mamillata Hance safflower anthocyanins.Therefore,simply providing the green Ardisia mamillata Hance with a growth environment similar to that of the red Ardisia mamillata Hance,which is beneficial to the accumulation of anthocyanins,does not allow the green Ardisia mamillata Hance to accumulate anthocyanins quickly,thereby achieving the purpose of changing the color of the Ardisia mamillata Hance.If we want to get a deeper understanding of the synthesis secrets of anthocyanins from Ardisia mamillata Hance,will need more in-depth research.