Effect Of Compound Chinese Herbal Medicine On Muscle Soreness Of Horses After Transportion

With the rapid development of the horse industry in Xinjiang,the transportation activities of horses across the regions have increased.What has become one of the most urgent problems to be solved in the development of horse industry is that the declination in function of the horses and their disease resulting from being transported.In this study,the Xinjiang Yili horses were selected as research targets,and the acute toxicity test of compound Chinese herbal medicine granules in mice and the test of alleviation of horse muscle soreness caused by transportation were performed.1.The preparation of compound Chinese herbal medicine granules and safety assessment in mice.The optimal ratio of auxiliary materials to extracts was screened out according to comprehensive analysis of granulation evaluation and molding rate measurement.The traditional Chinese medicine compound granules were prepared by wet method;the inspection standards of granulation quality were carried out according to the guideline and procedure specified in Chinese Pharmacopeia.Results:the optimal ratio to extracts and auxiliary materials is as follows,extracts:dextrin:sucrose(1:2:2).The size of the traditional Chinese medicine compound granules is 24 mesh,and the color is light brown.The granularity,dehydration standard,angle of repose,and solubility of the granules all meet the granulation standards of the Chinese Pharmacopoeia.Twenty-four SPF Kunming mice weighing 20±2 g were randomly divided into the control group(distilled water)and low,medium and high dose groups.The treatment groups were fed with boiled Chinese medicine by continuous gavage for 7 days.The mice were weighed on the 1st,3rd,and 5th day after gavage.The mice were sacrificed after the test,and the weights of the heart,liver,spleen,lung,and kidney were measured.After organ coefficients being calculated,histopathological sections were prepared and observed under a pathological microscope.The results showed that there were no difference in the light of MID,MID%and GRAN of mice in different dose groups in the experimental group,HGB showed a decreasing trend,but MPV and MCV showed an increasing trend;there was no significant difference in body weight and organ coefficients of each dose group;the liver,spleen,and lungs appeared mild inflammatory reactions in the high-dose group,but all were within the normal range;the granules were safe and non-toxic when the mice were given LD 50 at 15000 mg kg to 16000 mg/kg orally.2.The effect of the Chinese herbal medicine granules on alleviating the muscle soreness after transportation.Ten 24 month-old Yili mares with similar weight were randomly selected and divided into two groups.The control group was fed with basic diet and the experimental group was fed with Chinese herbal medicine granules+basic Diet.After the Yili mares were continuous bred for 5 days,the transportation test was performed.During the transportation,the food and water were prohibited,and the horses were transported with a total distance of about 380 km,which spent about 500 mins.The venous blood of the neck vein was collected to determine the relevant physiological,biochemical and hormonal indicators before transportation,and after the transportation of 4 h,8 h,and 12 h.The results showed that:(1)compared with the results before transportation,the MID,MID%,HGB,and SOD of the control group significantly(P<0.05)or extremely significantly reduced(P<0.01),but the GRAN%,MCV,SD,LD,MDA,IL-6 of the control group significantly(P<0.05)or extremely significantly increased(P<0.01),and Ca2+,CK,UREA of the control group and so on had no significant changes after the transportation of 4 h and/or 8 h and/or 12 h.(2)Compared with the results before transportation,the HGB and SOD of the experimental group significantly(P<0.05)or extremely significantly reduced(P<0.01),but the MCV,MPV,LD,LDH,MDA,IL-6 of the experimental group significantly(P<0.05)or extremely significantly increased(P<0.01)after the transportation of 4h and/or 8h and/or 12 h.(3)Compared the result with before the transportation of 4h,the increase range of LD and SOD of the experimental group significantly(P<0.05)or extremely significantly reduced(P<0.01),but the increase range of Ca2+ significantly(P<0.05)or extremely significantly increased(P<0.05)before transportation,after the transportation of 4 h and 8 h,and after the terminal transportation of 12 h.Compared with the control group,the UREA,IL-6,LDH,UREA,MDA and CK of the experimental group had no significant difference at each point of time during transportation.The results show that:1.The optimal ratio of extract and auxiliary materials is as follows:extract:dextrin:sucrose(1:2:2).The granularity,dehydration standard,angle of repose,and solubility of the granules all meet the granulation standards of the Chinese Pharmacopoeia.The size of granules had no significant effect on the blood indexes of mice,and there were no significant changes in body weight and organ coefficients.No pathological changes were observed in the pathological sections.The mice were given LD 50 at 15000 mg/kg to 16000 mg/kg orally,which suggested that the granules are safe and non-toxic.It shows that the Chinese herbal medicine prescription has no toxic and side effects.2.The relevant indexes LD,SOD,IL-6 and LDH of muscle soreness of horses caused by transportation have no changes.Most of the indexes begin to change after the transportation of 4 h.Chinese medicine granules can suppress the increase of LD and IL-6.The body’s ability to metabolize LD can be enhanced by increasing the content of LDH,which indicates it has the effect of alleviating muscle soreness after horses being transported.