Effects Of Neurodynamic Mobilization On Lower Limb Movement And Inflammatory Related Factors In Rabbit Model Of Chronic Constriction Injury

Objective:To observe the behavioral score of rabbit chronic constriction injury model treated by neurodynamic mobilization,the expression of TNF-αin injured sciatic nerve trunk,the expression of miR-124 and CGRP in DRG on the compressed side of sciatic nerve,the expression of AQP4 in spinal cord dorsal horn and PGE₂in blood.To explore the effect of neurodynamic mobilization on the motor function of lower extremities in rabbits with chronic sciatic nerve constriction injury and the regulation mechanism of inflammatory related factors,so as to provide theoretical basis for neurodynamic mobilization in the treatment of lower limb dysfunction caused by peripheral nerve injuries.Methods:72 healthy adult male Japanese big-eared rabbits were randomly divided into normal group,sham operation group,CCI model group and NM（CCI+NM）group,with 18 rabbits in each group.Each group was divided into3 subgroups at 1 week,2 weeks and 3 weeks after successful modeling,with 6rats in each group（The normal group did not make a model,and the time was the same as that of the other three groups）.The normal group did not do any treatment,the sham operation group only exposed the sciatic nerve without ligation,and the model group did not do any treatment after successful of CCI modeling.The above three groups were fed in a single cage and strengthened for 3 weeks.After 7 days of successful modeling of CCI model in NM group,according to NM treatment,the rats were treated 6 days a week for 10 minutes each time for 2 weeks.All groups were sampled after behavior score at 1 week,2 weeks and 3 weeks after successful modeling.The expression of TNF-αand PGE₂was detected by ELISA,and the expression of miR-124,CGRP and AQP4 was detected by q RT-PCR.Results:1 Toe reflex and modified Tarlov score（1）The Toe reflex and modified Tarlov score in the model group and the NM group were lower than those in the blank group（P<0.05）,the model is successful.There was no significant difference between the model group and NM comparison（P>0.05）.（2）After 2 weeks of modeling（NM intervention for 1 week）,the scores of the NM group were both higher than that of the model group,but there was no significant difference（P>0.05）,NM intervention temporarily failed to improve the lower limb function score of CCI rabbits.（3）After 3 weeks of modeling（NM intervention 2 weeks）,the NM group scores were higher than the model group（P<0.05）.2 Expression of PGE2 in blood（1）At the time point of 1,2 and 3 weeks after modeling,the serum PGE₂concentration of rabbits in the model group and NM group was increased compared with that in the blank group and the sham operation group,with statistical significance（P<0.05）.（2）At the time point 2 weeks after modeling,the serum PGE₂concentration of rabbits in NM group was decreased compared with that in model group,but there was no statistical significance（P>0.05）.（3）At the time point 3weeks after modeling,the serum PGE₂concentration of rabbits in NM group was significantly lower than that in model group,with statistical significance（P<0.05）.3 Expression of TNF-αin sciatic nerve（1）At 1 week and 2 weeks after modeling,serum TNF-αconcentrations in the model group and the NM group were increased compared with the blank group and the sham group,with statistical significance（P<0.05）.（2）At the time point of 2weeks after modeling,the serum TNF-αconcentration of rabbits in NM group was decreased compared with that in model group,but there was no statistical significance（P>0.05）.（3）At the time point of 3 weeks after modeling,the serum TNF-αconcentration of rabbits in NM group was significantly lower than that in model group（P<0.05）.4 Expression of AQP4 in spinal dorsal horn（1）At the time point of 1 week after modeling,the expression of AQP4 in DH tissue of rabbits in the model group and the NM group was increased compared with that in the blank group and the sham group,but the expression was not statistically significant（P>0.05）.（2）At the time point of 2 weeks after modeling,the expression of AQP4 in DH tissue of rabbits in NM group was decreased compared with that in model group,but there was no statistical significance（P>0.05）.（3）At the time point 3 weeks after modeling,the expression of AQP4 in DH tissue of rabbits in the NM group was not statistically significant compared with that in the blank group,sham operation group and model group（P>0.05）.5 Expression of CGRP in DRG（1）At the time point one week after modeling,the expression of CGRP in DH tissue of rabbits in the model group and NM group was increased compared with that in the blank group and the sham operation group,with statistical significance（P<0.05）.（2）At the time point 2 weeks after modeling,the expression level of CGRP in DH tissue of rabbits in NM group was higher than that in blank group and sham group,and lower than that in model group,but there was no statistical significance（P>0.05）.（3）At 3 weeks after modeling,the expression level of CGRP in DH tissue of rabbits in the NM group was significantly lower than that in the model group,with statistical significance（P<0.05）.6 Expression of miR-124 in DRG（1）At 1,2 and 3 weeks after modeling,there was no statistically significant difference in the expression of miR-124 in the DRG tissues between the model group and the NM group compared with the blank group and the sham group（P>0.05）.（2）The pairwise comparison of miR-124 expression in the model group at 1 week,2weeks and 3 weeks after modeling showed no statistical significance compared with the blank group at the same time period（P>0.05）.Conclusion:1.Neurodynamic mobilization can improve the motor function of lower limbs in rabbits with chronic sciatic nerve compression injury.2.Neurodynamic mobilization reduces the expression of inflammatory related factors and neurotransmitters such as prostaglandin E₂,tumor necrosis factor-αand calcitonin gene-related peptide in rabbit models of chronic constriction injury model.It may play a positive role in the improvement of lower limb motor function in rabbits with sciatic nerve injury.