The Research Of Quality Standard And Concentarted Herb Granule Of Galii Aparinis Herba

Galii Aparinis Herba belongs to Galium aparine L.var.tenerum(Gren.et.Godr.)Rchb.The whole dry grass has been commonly used frequently for its cations such as detoxification,diuretic swelling,hemostasis,for the treatment of edema,urinary tract infection,dysentery,blood in the stool blood,fall injury,carbuncle swollen boils,insect snake bites.As the quality standard of Galii Aparinis Herba is not perfect,subsequent promotion and application of the medicine is limited severely.Therefore,the standard of Dioscorea opposita was included in the "Hebei medicinal standard"(2016)project,This article aims to establish and improve the quality standard of Galii Aparinis Herba,and provide the basis for the comprehensive and effective control of the quality of Galii Aparinis Herba.Galii Aparinis Herba is included in the "Hebei medicinal standard"(2016 edition),and the microscopic identification,thin layer identification,content determination,examination items and leaching materials of medicinal materials were studied,the microscopic characteristics of the powder of Galii Aparinis Herba were determined.Thin layer chromatography method was established for the identification of Galii Aparinis Herba.The powder of the medicinal material is refluxed by heating with methanol and the filtrate is concentrated to extract the ethyl acetate layer and the n-butanol layer extract.The ethyl acetate layer was pointed to silica gel G thin layer plate,developing system chloroform-methanol-water(8:2:0.2)was applied,and observed under UV light at 365 nm.The n-butanol layer was washed with 10%sulfuric acid and ethanol solution at 105℃until the color was clear with ethyl acetate-formic acid-water(12:3:1)as the developing solvent.The same color spots in the TLC graphs of sample existed at the corresponding position compared with the controls.The HPLC method was established for the determination of chlorogenic acid.The mobile phase were acetonitrile and 0.1%phosphoric acid(13:87);the fiow rate was 1.0 mL-min-l;the detection wavelength was 327 nm;the column temperature was 25℃;the injection volume was 20 μL.The moisture content,ash(total ash and acid-insoluble ash content)and extract of medicine were determined.Moisture was not more than 13.0%;total ash was not more than 16.0%;acid-insolubal ash was not more than 3.5%;and alcohol extracts was not less than 7.0%.According to the traditional decoction with a large dose,poor quality stability,boiling,taking,carrying inconvenience and other characteristics,so it made advanced technology,stable quality control,accurate dose,easy to take,easy to carry,safe and effective calyx compound formula is of great significance.So to study the preparation process and quality standard of the compound granule of Galii Aparinis Herba,for the scale production and application lay the foundationThe paramerers of Galii Aparinis Herba extraction and concentration process were optimized.The preparation process of the granules of the compound granule of Galii Aparinis Herba was determined by using the rate of extraction and chlorogenic acid transfer rate as an index.Soaking for 30min,extract two times,the first time the amount of water is 15 times,the second time the water is 12 times,each time 60min;Concentrated method for the concentration of decompression,drying method for spray drying.Through the identification of the thin layer,the determination of the content,the inspection items and the leaching materials of the test samples,the quality standard of the controllable granule was established.