Study On The Histological Inflammatory Clinical And The Proteomic Study Of Benign Prostatic Hyperplasia

Objective1.To investigate the relationship between histological inflammation and the clinical symptoms of benign prostatic hyperplasia(BPH).2.The proteomic technology was used to select the differentially expressed proteins in prostate tissue samples from BPH combined with histological inflammation and BPH alone,providing new clues for the molecular basis of the occurrence and progress of BPH,and searching for the new potential biomarkers for BPH.Method1.The clinical data of 196 BPH patients who underwent transurethral resection of the prostate were collected.According to the results of HE staining of patient tissue simples,the patients were divided into BPH combined with histological inflammation group and BPH alone group.Differences in acute urinary retention(AUR),prostate-specific antigen(PSA),prostate volume(PV),quality of life(QoL)scores,maximum urine flow rate(Qmax),post-void residual urine volume(PVR),testosterone(T),estradiol(E2),E2/T values,International Prostate Symptom Score(IPSS)and its sub-scores were compared between the two groups.The SPSS software version 25.0 was used for statistical analysis.2.Nine cases of BPH tissue samples with histological inflammation were selected,including 3 cases each of the mild,moderate and severe inflammation groups.At the same time,3 cases of simple BPH tissue specimens were selected as the control group.Using proteomic technology to identify the differentially expressed proteins between each inflammatory group and the simple BPH group,and bioinformatics analysis was used on these differentially proteins.Results1.The clinical study found that among 196 patients with BPH,42 cases(21.4%)were in the BPH alone group,and 154(78.6%)were in the BPH combined with histological inflammation.The results demonstrated that the incidence of AUR,PV,IPS S total scores,IPS S-storage scores and QOL scores in BPH combined with histological inflammation group were higher than those in BPH alone group(P<0.05).But there were no significant differences in PSA,IPS S-voiding scores,PVR,Qmax,T,E2 and E2/T values between the two groups(P>0.05).2.The proteomic analysis indicated that there were 332 proteins differentially expressed between the BPH combined with histological inflammation group and the simple BPH group,and 6 overlapping proteins were identified from the 3 groups.Among them,Radixin(RDX),High mobility group Protein B2(HMGB2),Diphosphoinositol polyphosphate phosphohydrolase 2(NUDT4),and Actin filament-associated protein 1-like 2(AFAP1L2)were up-regulated.GPI-anchor transamidase(PIGK)and Purkinje cell protein 4(PCP4)were down-regulated.3.GO analysis implied that these differential proteins were mainly concentrated in cells,organelles,membrane and extracellular regions,and their main molecular functions were catalytic and binding activity,and they were related to biological regulation,cellular and metabolic processes.In addition,the protein interaction network analysis demonstrated that the six proteins have certain connections with other proteins,of which HMGB2,NUDT4 and RDX were closely related to other proteins,while PIGK,PCP4 and AFAP1L2 were weakly associated with other proteins.Conclusions1.The study showed that most BPH patients had histological inflammation,and patients with histological inflammation had greater PV,more severe LUTS,and higher risk of AUR.Prostatic histological inflammation is closely related to BPH,which may be a key factor in the occurrence and clinical development of BPH.2.In this study,six differentially expressed proteins including HMGB2,NUDT4,RDX,AFAP1L2,PIGK and PCP4 were identified in BPH prostate tissues by the proteomic technique.Among them,the expression of HMGB2,NUDT4,RDX andAFAP1L2 were up-regulated,while the PIGK and PCP4 were down-regulated.3.The Results of bioinformatics analysis indicated that HMGB2,NUDT4,RDX,AFAP1L2,PIGK and PCP4 may be the key proteins that play an important role in BPH,and may be potential biomarkers of BPH.