Study On The Relationship Between Interferon-induced Transmembrane Protein3 Genetic Polymorphism And Outcome Of HBV Infection

Objective:By analyzing the distribution of C/T polymorphism at gene rs12252 of interferon induced transmembrane protein 3(IFITM3),to explore the relationship between IFITM3 gene polymorphism and the susceptibility of hepatitis B virus infection,to explore the relationship between IFITM3 rs12252 genotypes and HBV related cirrhosis and HBV related liver cancer,and to reveal the host genetic factors that affect the pathogenicity of hepatitis B virus.Methods:The serum samples of 159 patients were collected from the department of Infectious Diseases,Interventional Therapy and Hepatobiliary Surgery of the Second Clinical Medical College of Jinan University(Shenzhen People’s Hospital)from January 2018 to December 2019.There were 130 cases of HBV-related liver diseases(the hepatitis B group),including 68 cases with HBV-related liver cancer(the liver cancer group),30 cases with HBV-related cirrhosis(the cirrhosis group)and 32 cases with HBV-related chronic hepatitis(the non-cirrhosis group),and 29 cases of control(the control group).They are all Han people,mainly from South China.The collected peripheral blood was stored in a refrigerator at-80°C.The clinical laboratory results of GPT,GST,GGT,ALP,albumin,total bilirubin,prothrombin time,prothrombin activity,AFP,HBV DNA and HBe Ag were collected.The DNA of peripheral blood was extracted,the corresponding target fragment was amplified and sequenced,and the SNP at rs12252 of IFITM3 gene was detected.SPSS version 22.0 software was used to analyze the results.X~2test was used to compare the distribution differences of IFITM3 rs12252 genotype frequency and allele frequency among the groups;Goodness of fit test was used to analyze whether the control group was consistent with Hardy Weinberg balance law.P<0.05 was statistically significant.Results:(1)The control group was in accordance with Hardy Weinberg equilibrium law.(2)There was no significant difference between the hepatitis B group and the control group in the frequency of IFITM3 rs12252 CC/CT/TT genotype and C/T allele.(3)There were no significant differences in C-dominant inheritance,C-recessive inheritance,C/T codominant inheritance,T-dominant inheritance and T-recessive inheritance among the control group,hepatitis B infection group,chronic hepatitis B group,HBV related cirrhosis group and HBV related liver cancer group.(4)Compared with other groups,the OR value of genetic model with IFITM3 rs12252C allele in cirrhosis group was more than 1.(5)Compared with other groups,the OR value of genetic model with IFITM3 rs12252 T allele in HCC group was more than 1.(6)There was no significant difference in IFITM3 rs12252 genotypes between the high-load and low-load HBV DNA group(X~2=0.57,P=0.75).There was no significant difference in IFITM3 rs12252 genotype between HBe Ag positive group and HBe Ag negative group(X~2=0.26,P=0.88).Conclusion:(1)The control group was in accordance with Hardy Weinberg equilibrium law,the control group met the standard.(2)No relationship was found between human IFITM3 rs12252 C/T polymorphism and the susceptibility of HBV infection and the occurrence of HBV related cirrhosis and liver cancer in this study.(3)Human IFITM3 rs12252 C/T allele may not be involved in the host regulation of hepatitis B virus replication in this study.