The Relationship Between The Single Nucleotide Polymorphisms Of IL-22,IL-22R1,IL-27 Genes And The Susceptibility Of Oral Lichen Planus

ObjectiveTo explore the correlation between the single nucleotide polymorphisms of IL-22,IL-22R1,IL-27 genes and the susceptibility of oral lichen planus in the Han population in Qingdao,China.MethodsThis study was a case control experiment,the genotype and allele frequency of IL-22 rs2227485,IL-22R1 rs3795299,IL-27 rs153109 were detected by Taqman real-time PCR in 108 oral lichen planus patients(including 31 cases of erosive oral lichen planus and 77 cases of nonerosive oral lichen planus)and 118 healthy control subjects of han population in Qingdao,China.Furthermore,the analysis of the correlation and the evaluation of relative risk degree were carried out.Results:1.IL-22 rs2227485(1)The three genotypes of AA,AG and GG were detected in eOLP group,neOLP group,OLP group and control group.AG heterozygote was the most common genotype while the frequency distribution of G allele was the highest among the four groups.(2)There was no obvious difference in genotype and allele frequency among all groups(P>0.05).2.IL-22R1 rs3795299(1)CC,CG and GG genotypes were detected in eOLP group,neOLP group,OLP group and control group.CG heterozygous genotype was the most common genotype in eOLP group,neOLP group andOLP group,followed by GG genotype,while GG homozygous genotype was the most common genotype in control group,followed by CG heterozygous genotype.The frequency distribution of G allele was higher than C allele in the four groups.(2)Compared with the control group,the genotype and allele frequency distribution of IL-22R1 rs3795299 were statistically different in eOLP group,neOLP group andOLP group(P<0.05).Compared with the control group,the frequency of G allele was obviously lower in the eOLP,neOLP andOLP groups,while the frequency of C allele was significantly increased.(3)The association analysis of the relative risk showed that people with GG genotype had the 2.957 times higher risk of developing eOLP than those carrying CC+CG genotype(X~2=6.774,P=0.014<0.05,OR=2.957,95%CI=1.280-6.832),and people with C allele had the 2.172 times higher risk of developing eOLP than those with G allele(X~2=6.831,P=0.011<0.05,OR=2.172,95%CI=1.205-3.916).The relative risk of neOLP in the population with CC+CG genotypes was 1.999 times higher than that with GG homozygote(X~2=5.396,P=0.026<0.05,OR=1.999,95%CI=1.111-3.597).The relative risk ofOLP in the population with CC+CG genotypes was 2.232 times higher than that with GG genotypes(X~2=8.755,P=0.003<0.05,OR=2.232,95%CI=1.307-3.814),and the risk ofOLP in the population with C allele was 1.688times higher than that with G allele(X~2=6.242,P=0.016<0.05,OR=1.688,95%CI=1.117-2.550).3.IL-27 rs153109(1)The three genotypes of AA,AG and GG were detected in eOLP group,neOLP group,OLP group and control group.AG heterozygote was the most common genotype while the frequency distribution of A allele was the highest among the four groups.(2)It was not obviously different in genotype and allele frequency among all groups(P>0.05).Conclusion1.There were genetic polymorphisms at IL-22 rs2227485,IL-22R1 rs3795299 and IL-27 rs153109 in the Han population in Qingdao,China,and three genotypes could be detected.2.The C allele of IL-22R1 rs3795299 may be susceptible toOLP,and the G allele may exist as a protective factor forOLP.3.The single nucleotide polymorphisms of IL-22 rs2227485 and IL-27 rs153109 may be unrelated to the incidence and development ofOLP.