| ObjectiveThrough the inverse gravity to climb the establishment of an index of the movement for fruit flies anti-aging model,and from flies life,heart failure rate,ultrastructure and fruit fly heart tube enzymes and antioxidant.enzymes activity of a few respects study movement on the fly,the life of the heart function ’and the influence of aging,at the same time,increasing movement of the effects of aging delay fruit flies evaluation.Material and methodsFruit flies wild type W1118 strains of a tube(American Birmingham institute provided),expand the training within 24h after collecting 3600 only then feathered male fruit flies,and randomly divided into six groups:blank control,1.5h exercise group,2h exercise group,2.5 h exercise group,3h exercise group,3.5h exercise group,600 only/group,20 only/tube,25℃ constant temperature,50%humidity,12 hours day/night cycle raising.The platform movement(fruit flies platform movement device is independent research and development),8 day old executive sports programs to climb index as fruit flies athletic intensity monitoring programme.Using video detection methods,to climb index-time curve graph making observations to climb the ability to fly.The flies is divided into six groups:blank control,1.5h exercise group,2h exercise group,2.5 h exercise group,3h exercise group,3.5h exercise group,starting rate for 24 seconds/turn.Fruit flies movement plan:the first stage:the environment to adapt,collect within 24h provided the first male fruit flies seven days in the cultivation of the box raise not training;The second stage:to adapt to the platform of training;The third stage:the intensity of each day with provisions increasing load movement.Specific as follows:the first week speed for 24 seconds/turn,time is 2h.According to increase movement after time not increase the principle of exercise intensity were increasing,sports time increase rate for 15 min,every load lasts for a week,every week training 5 times,until the end of the experiment.2 p.m.every day 30 began to exercise,Saturday and Sunday off.ResultsThe results of Fruit flies athletic intensityThe detection six groups of fruit flies(each group of 100 only)are climbing index with increase of age and lower,but in the entire movement in the process of the scheme,2h exercise group and 2.5h exercise group climbing ability basically all quiet on the control group;The first week of movement,the second week,1.5h exercise group,2h exercise group,2.5h exercise group,3h exercise group,3.5h exercise group climbing index and blank control group compared to no great changes,From the third week of sports we can see 2.5h exercise group of fruit flies climb index significantly greater than all other categories.The results of Fruit flies life cycleBlank compared with controls,2.5h exercise group life expectancy increased(p<0.01),and 1.5h exercise group,2h exercise group,3h exercise group,3.5h average life expectancy in the exercise group did not change significantly;And compared with blank,3.5h half of the exercise group time of death shortened(p<0.05),and 1.5h exercise group,2h exercise group,2.5h exercise group,3h half of the exercise group time of death did not change;And blank compared with controls,1.5h exercise group,2h exercise group,2.5h exercise group,3h exercise group of fruit flies highest life increased(p<0.01),and 3.5h the highest life in the exercise group has not changed.And blank compared with controls,1.5h exercise group,2h exercise group,2.5h exercise group,3h exercise group,3.5h exercise group of fruit flies average rate were 1.36%and prolong its life,2.87%,5.86%,2.80%,-0.85%The results of Fruit flies heart functionWe select 31 day old heart function testing fruit fly found,blank control,1.5h exercise group,2hexercise group,2.5h exercise group,3h exercise group,3.5h exercise group fruit flies heart failure rate were 56%,58%,52%,46%,57%,69%.Then,six groups of fruit flies to 25℃constant temperature,put 50%humidity,12 hours a day/night cycle environment breeding to 38 day old again only select 100 each heart function test,the obtained result for:blank control,1.5h exercise group,2 h exercise group,2.5h exercise group,3h exercise group,3.5h exercise group fruit flies heart failure rate were 71%,68%,65%,62%,74%,80%.Through the 31 days of age and 38 day old fruit flies heart failure rate we found,for 3 weeks to exercise every day 1.5h,2h,2.5h of fruit flies incidence of heart failure compared to the control group fruit flies blank has dropped,especially the 2.5h exercise group fruit flies heart failure rate dropped significantly,and 3.5h exercise group heart failure rate and fruit fly blank significantly higher compared with controls.The result of HE dyeing and microscopic image analysis in flies Heart tubeYoung heart muscle control group fruit flies tube aligned,did not see the muscle fiber fracture,vice myocardial cell size,form the basic consistent,uniform chromatin,did not see the mast,necrosis and atrophy wait for a phenomenon.Old heart muscle fiber tube control group fruit flies atrophy and fracture appear,vice myocardial,cell number decreased,the cell size,shape is differ,the nucleus increases along with nuclear dissolve phenomenon,and have cell necrosis and atrophy wait for a phenomenon,obviously different.Older the exercise group heart flies the local myocardial fiber increased thick,also appeared appear atrophy and fracture,but no control group obvious middle-aged,vice myocardial cell size,shape is differ,and a cell nucleus,the phenomenon such as increasing atrophy,mild alien.The SOD immunohistochemical and microscopic image analysis results of flies heartAnd the young compared to the control group is very positive for middle-aged express significant reduce(P<0.01),old exercise group were significantly reduce positive expression(P<0.05);And old compared to the elderly in the exercise group positive expression is very significant increase(P<0.01).The MDA immunohistochemical and microscopic image analysis results of flies heartAnd the young compared to the control group is very positive for middle-aged express significant increase(P<0.01),old exercise group were significantly reduce positive expression(P<0.05);And old compared to the elderly in the exercise group positive expression is very significant increase(P<0.01).Conclusion(1)For 3 weeks,exercise every day 2h-2.5h can improve fruit flies with increase of age and reduce the climbing ability,resistance increased age sex recession;(2)For 3 weeks,exercise every day 1.5h、2h、2.5h can reduce the occurrence of fruit fly heart failure,thus effectively delaying cardiac function in the age of degradation,and extend the life of flies;(3)The organization of the heart flies the morphological observation and immunohistochemical tests showed that:through the exercise every day 2h-2.5h for 3weeks of the regular exercise can reduce the fruit flies myocardial cell apoptosis,which can achieve delay and improve fruit flies the degenerative change heart tube.(4)Can be used in a 2～2.5h movement model to establish fruit flies movement anti-aging model... |
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