Effects Of Diosgenin Extract Of Pansandra On Bcl-2/Bax Protein Expression In The Pancreas Of Type 1 Diabetic Mice

Purpose:The research method of network pharmacology was used to predict the active components,action targets and signal pathways of Discorea nipponica Makino,to explore the potential mechanism of action of active components of Discorea nipponica Makino on type 1diabetes,and to lay a theoretical foundation for future zoological experiments.According to the results of network pharmacology prediction,the main component of Chinese medicine Discorea nipponica Makino,dioscin was selected for animal experiments,and the effect of Dioscin on the expression of Bcl-2 and Bax protein in the pancreas of type 1 diabetic mice was further studied to further confirm the traditional Chinese medicine.Dioscin from Discorea nipponica Makino extract has a repairing effect on the pancreas of type 1 diabetic mice,and provides new treatment ideas for the prevention and treatment of type 1 diabetes by Chinese medicine.Materials and methods:1.the Network Pharmacology researchThrough database search,the domestic and foreign documents are sorted out and referenced,and the information of the main chemical components contained in the traditional Chinese medicine Discorea nipponica Makino is collected.According to the 5-fold principle of Lipinski drug properties,combined with domestic and foreign literature data,the effective active ingredients of the traditional Chinese medicine Discorea nipponica Makino are screened.The Swiss database was used to predict the targets of potential active ingredients in the traditional Chinese medicine Discorea nipponica Makino.Use OMIM,Genecards database to obtain disease targets.Use the Venny2.1 online software mapping tool platform to draw the Venn diagram to obtain the common drug-disease target;use the Cytoscape 3.7.2 software to construct the "drug-component-target-disease" network diagram;use the String10.5 database and Cytoscape 3.7.2 software draws PPI protein interaction network diagram,based on PPI protein interaction network diagram for topological analysis and cluster analysis;R software uses Bioconductor biological information software to perform GO biological process and cell component enrichment on the obtained target protein Set analysis,enrichment analysis of KEGG signal pathway.2.Animal experiments89 SPF-grade adult male C57BL/6 mice were selected for 12 hours of light cycle and free access to food and water.The mice were adapted to normal feeding within 7 days,and 12 mice were randomly selected as the normal control group.All mice were fed with normal diet.After 12 hours of complete fasting for the remaining mice,the remaining mice were injected intraperitoneally for 5 consecutive days.The injection was 1% streptozotocin 50mg/kg(The p H value is 4.2),the tail vein blood of the mouse is collected with a blood needle,and the blood glucose is measured.The value>16.7mmol/L is judged as successful in inducing the type 1 diabetes model.After modeling,the remaining mice were randomly divided into groups,and they were labeled as type 1 diabetes model group,low-dose diosgenin group,high-dose diosgenin group,and prednisone group.Observe the general condition(including coat color,mental state,behavioral characteristics,etc.)of mice in each group at 0w before treatment,4w,8w,and 12 w after treatment,blood glucose,and observe 0w' before modeling,4w,8w,and 12 w after treatment.Mice in each group were sacrificed at the end of treatment for 12 weeks,and their pancreatic tissues were taken for future use.Western Blot(WB)was used to detect the expression levels of Bcl-2 and Bax protein in the pancreatic tissue of each group of mice.Results:1.Twenty-seven active components of Discorea nipponica Makino can play a role in the treatment of T1 DM by participating in cell apoptosis,immunity,oxidative stress and other processes.2.Diosgenin can up-regulate the expression of Bcl-2 protein in the pancreas of T1 DM mice,down-regulate the expression of Bax protein in the pancreas of T1 DM mice,improve the cell apoptosis in the pancreas of T1 DM mice,and thus protect the pancreatic ? cells of T1 DM mice.Conclusions:1.Bcl-2/Bax signaling pathway is one of the pathways of islet ? cell apoptosis in T1 DM mice.2.Dioscin may improve the pancreatic cell apoptosis of T1 DM mice by up-regulating the level of Bcl-2 protein and down-regulating the level of Bax protein,and play a role in protecting pancreatic ?-cells in T1 DM mice.