Studies On Chemical Constituents And Biological Activities Of Invasive Plants Ambrosia Artemisiifolia And Solanum Rostratum

With the rapid development of economic globalization and trade among countries,plant invasion has become one of the key problems facing the balanced and stable development of the global ecosystem,and the serious ecological and economic problems caused by this have attracted extensive attention of people all over the world.In the past,chemical pesticides and mechanical eradication were used to control invasive plants,but these methods were time-consuming and laborious,and polluted the environment.In recent years,more and more people have found that invasive plants also contain abundant active chemical components,and making full use of these resources is also an effective way to control them.Therefore,this study investigated the chemical components and biological activities of two typical invasive plants Ambrosia artemisiifolia and Solanum rostratum in northern China,in order to control these two invasive plants from the perspective of resource development and utilization.It is expected to obtain the lead active substances with the prospect of application in plant pesticides.Ambrosia artemisiifolia,an annual herb of the genus Ambrosia(Compositae),was introduced into China in the early 1930 s,and has now spread widely in northeast China.It is highly invasive and has caused great harm to natural vegetation,agricultural production and human health,and has become an exotic malignant weed that needs to be controlled urgently.In this study,five compounds(A1-A5)were isolated from the ethanol extract of A.ambrosia by different column chromatography.Their structures were identified by 1D-NMR,2D-NMR,HR-ESIMS and calculated ECD,and all of them were new compounds.The phytotoxic activities showed that compound A1 significantly inhibited the root growth of Arabidopsis thaliana,and compounds A2-A5 promoted the root growth to some extent.Solanum rostratum is an annual herb of Solanum(Solanaceae).This plant originated from North America,and has now spread to Xinjiang,Jilin,Hebei,Shanxi,Beijing and Inner Mongolia,and the trend of spread is still expanding,Which has damaged the ecological environment and biodiversity of the invasive areas seriously.In this study,eight compounds(B1-B8)were isolated from the ethanol ethanol extract of S.rostratum by different column chromatography.Their structures were identified by 1D-NMR,2D-NMR,HR-ESIMS,calculated ECD Antifeedant activities showed that B1-B8 exhibited different degrees of inhibition against Helicoverpa armigera,among them compound B1 and B4 had the significant antifeedant activities.In vitro antifeeding related enzyme activities showed that compounds B1 and B4 could inhibit Ach E and Car E.In addition,molecular docking simulation was used to explore the binding sites and action modes of compounds B1 and B4 with the above two enzymes.Ambrosia artemisiifolia and Solanum rostratum are highly invasive and malignant weeds.In this study,we explored the successful invasion mechanism of Ambrosia artemisiifolia and Solanum rostratum and tried to develop and utilize them to improve the quality of human life and provide lead active substances for the development of natural plant-derived pesticides.