Identification And Expression Analysis Of The SULTR Gene Family In Tomato

Sulfate transporter(SULTR)is responsible for sulfate transport and uptake and plays a critical role in plant growth and development and adaptation to adversity.To investigate the regulatory network involved in SULTR genes,this study identified the tomato SULTR gene family and analyzed its phylogenetic tree,physicochemical properties,predicted subcellular localization,chromosomal localization,gene structure,conserved motifs,cis-acting elements,predicted protein secondary structure,predicted protein phosphorylation sites,predicted protein interactions,tissue specificity,different hormone treatments and expression of Pst DC3000 treatment.The main findings are as follows.1.A total of 15 SULTR gene family members were identified in the tomato genome,which can be divided into four subfamilies.They were distributed in only seven of all tomato chromosomes,with the largest number on chromosome 12 with five SULTR genes.Ten of the conserved motifs were predicted to contain highly conserved motifs.Nine SULTR genes were found to contain 12 exons and 11 introns in the gene structure analysis;the most unusual one was Sly SULTR3;4b with only one exon.Covariance revealed five homologous gene pairs within species;interspecies covariance revealed 12 direct homologous gene pairs in tomato and Arabidopsis: two direct homologous gene pairs in tomato and rice.Cis-element prediction revealed multiple cis-acting elements in the promoter region of Sly SULTR involved in phytohormone and stress response to adversity.2.Analysis of the expression patterns of tomato SULTR gene family members in different tissues.The results showed that most of the genes in the transcriptome data were expressed in roots and flowers,with Sly SULTR4;1 being more specific having high expression in several sites.q RT-PCR results were increased for stems,but fruits were not subdivided into periods.Twelve genes were detected with expression signals in all tissues,and Sly SULTR3;4b had the highest expression in fruit.3.qRT-PCR was used to analyze the expression of SULTR genes in leaves treated with abscissa acid(ABA),ethylene(ETH),methyl jasmone(JA),and salicylic acid(SA).The results showed that the expression of Sly SULTR1;1b increased to the highest level after 6 h of abscisin acid treatment;Sly SULTR3;4a increased to the highest level after 2 h of ethylene treatment;Sly SULTR2;1 increased to the highest level after 2 h of jasmonic acid treatment;Sly SULTR1;1b increased to the highest level after 2 h of salicylic acid treatment,and they were all positively regulated by the hormone treatments.All of them were positively regulated by hormone treatment.4.In tomatoes,the responses of eight SULTR genes were examined under Pst DC3000 treatment,and three were found to be positively regulated with the highest response at 24 h.The treatment of tomatoes with Na HS as an S donor was found to improve the disease resistance of tomato leaves to some extent.