Research Effect Of Feeding Mulberry Leaf Extract To Yellow Feather Broilers

Mulberry leaf extract contains rich active ingredients,including flavonoids,polysaccharides and polyphenols,which are of great benefit to the health of livestock and poultry.This experiment took yellow feather broilers as the research object,through in vitro and feeding experiments,in order to provide theoretical basis and production guidance for the application of mulberry leaf extract in broilers.Objective:Based on the development and utilization of new feed resources,this experiment was conducted to evaluate the prospect of mulberry leaf extract as a new feed additive in the diet of yellow feather broilers.Taking yellow feather broilers as the research object,and to synthesize the in vitro antioxidant activity of mulberry leaf extract and its effect on yellow feather broilers.Methods:This experiment was conducted to make a preliminary judgment for the antioxidant activity of mulberry leaf extract by studying its reducing ability,hydroxyl radicals;2,2’-bi-azobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt radicals and1,1-diphenyl-2-bitter hydrazine radical scavenging activity.120 42-day-old male medium speed yellow feather broiler were randomly divided into 4 groups with 6 repeats and 5 broiler for each repeat.Control group were fed the basal diet,group 0.05%,0.1%and 0.2%were respectively fed the basal diet supplemented with mulberry leaf extract 0.05%,0.1%and0.2%.The trial period was 42 days.Determining the effects of mulberry leaf extract on growth performance,slaughter performance,organ index,serum biochemical index,nutrient apparent digestibility and antioxidant capacity of yellow feather broilers.Results:(1)The mulberry leaf extract had a dose-dependent scavenging effect on hydroxyl radical,ABTS radical and DPPH radical in the range of 0.2-1.5 mg/m L.With the increase of the concentration of mulberry leaf extract,its reduction ability also increased.(2)Compared with the CON group,the consumed weight gain ratio(F/G)and liver index were significantly lower in the 0.1%and 0.2%groups(P<0.05),the average daily gain(ADG)was higher in each test group than in the CON group,and the abdominal fat rate and subcutaneous fat thickness were lower than in the CON group.(3)The addition of mulberry leaf extract to the diet had no significant effect(P>0.05)on any of the serum biochemical indexes in broiler chickens,with BUN,LDL-C and TC levels decreasing with the increase in the addition of mulberry leaf extract.The apparent digestibility of phosphorus(P)and crude protein(CP)in the 0.1%group was significantly higher than that in the CON group in each test group(P<0.05).(4)Compared with the CON group,serum GSH-P_X,serum T-AOC and liver GSH-P_X levels were significantly higher(P<0.05)and liver MDA,liver ROS and serum ROS levels were significantly lower(P<0.05)in each test group;liver T-AOC and serum CAT levels were significantly higher(P<0.05)in the 0.2%group than in the CON group;liver CAT content was significantly higher in the 0.1%and 0.2%groups(P<0.05).(5)Compared with CON,the relative expression of hepatic Nrf2 m RNA was significantly higher in the 0.1%group(P<0.05),the relative expression of SOD1 m RNA was significantly higher in the 0.2%group(P<0.05),the relative expression of hepatic CAT m RNA was significantly higher in the 0.1%and 0.2%groups(P<0.05),and the relative expression of hepatic GSH-P_X m RNA in all test groups was significantly(P<0.05),and the relative expression of liver GSH-P_X m RNA was significantly higher in all test groups(P<0.05).Conclusion:Mulberry leaf extract has good in vitro antioxidant activity and can significantly increase the antioxidant capacity of yellow feather broilers.Significantly improving yellow feather broilers growth and slaughter performance,as well as reduce the weight gain ratio and improve the apparent digestibility of crude protein and phosphorus.The appropriate dosage is 0.1%-0.2%.