Effects Of Genistein On Traumatic Proliferative Vitreoretinopathy In Rabbits

[Background]Proliferative vitreoretinopathy (PVR) is a reactive process of the ocular tissue after perforating trauma, retinal detachment, and surgical manipulations. It is characterized by retinal pigment epithelium cells, neuroglial, and inflammative cells proliferate on the retina, under the retina and in the vitreous cavity. Proliferative vitreoretinopathy (PVR) is still the most common cause of failure of surgery for rhegmatogenous retinal detachment, despite the substantial effort that has been devoted to better understanding and managing this condition during the past 25 years, With the better understanding of the pathological mechanism of PVR, many ophthalmologists bigan to search for effective medicine treatment for PVR.Gnistein is the main component of soybean isoflavone. In 1987, Akiyama reported that genistein is the inhibitor of tyrosine kinase frist. In resent years, many people focused on genistein because of it's effect on antitumor and antineovascularization. Many vitro experiments have proved that genistein can inhibit proliferation and induce apoptosis of retinal pigment epithelium cells, and provent retinal pigment epithelium cells from secreting many kinds of cytokines.Our study is to investigate the role of genistein in rabbit traumatic proliferative vitreoretinopathy and its influence on the expression of PDGF, bFGF, and PCNA, to provide theory proof for the medicine treatment of PVR.[Objective]1. To study the inhibition of genistein in traumatic proliferative vitreoratinopathy.2. To study the influence of genistein on expression of PDGF, bFGF, and PCNA in traumatic proliferative vitreoratinopathy model.[Method]1. Traumatic PVR models were induced in pigmented rabbits by intravitreal injection of platelet rich plasma. Models were divided in 5 groups: A group(negative control/DMSO 0.1 ml), B group(positive control/5-FU 1mg), C group(genistein 2μg), D group(genistein 20μg), E group(genistein 40μg). After surgery, the eyes were examined ophthalmoscopically on the 1st, 3rd,...